Electrospun Nanofibrous Mesh Based on PVA, Chitosan, and Usnic Acid for Applications in Wound Healing
Abstract
:1. Introduction
2. Results and Discussions
2.1. Fourier Transform Infrared Spectroscopy (FT-IR)
2.2. Scanning Electron Microscopy (SEM)
2.3. Stability Tests
2.4. XTT Assay
2.5. Glutathione (GSH) Assay
2.6. Fluorescence Microscopy
2.7. Effect of Nanostructured Meshes on Biofilm Production
3. Materials and Methods
3.1. Materials
3.2. Preparation Methods and Electrospinning Parameters
3.3. Physicochemical Characterization
3.3.1. Fourier Transform Infrared Spectroscopy (FT-IR)
3.3.2. Scanning Electron Microscopy (SEM)
3.3.3. Stability Tests
3.4. In Vitro Biocompatibility
3.4.1. XTT Assay
3.4.2. GSH-Glo Glutathione Assay
3.4.3. Fluorescence Microscopy
3.4.4. Anti-Biofilm Effect
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameters | Solutions | |
---|---|---|
5%PVA_2%CS | 5%PVA_2%CS_UA | |
Flow rate, mL/h | 3–7 | 5 |
Output voltage, KV | −6 -> −3 17 -> 20 | −6.5 17 |
Fan speed, rpm | 80 | 80 |
Humidity degree, % | ~20 | ~20 |
Temperature, °C | ~28 | ~28 |
Heater output, KW | 0.6 | 0.6 |
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Stoica, A.E.; Albuleț, D.; Bîrcă, A.C.; Iordache, F.; Ficai, A.; Grumezescu, A.M.; Vasile, B.Ș.; Andronescu, E.; Marinescu, F.; Holban, A.M. Electrospun Nanofibrous Mesh Based on PVA, Chitosan, and Usnic Acid for Applications in Wound Healing. Int. J. Mol. Sci. 2023, 24, 11037. https://doi.org/10.3390/ijms241311037
Stoica AE, Albuleț D, Bîrcă AC, Iordache F, Ficai A, Grumezescu AM, Vasile BȘ, Andronescu E, Marinescu F, Holban AM. Electrospun Nanofibrous Mesh Based on PVA, Chitosan, and Usnic Acid for Applications in Wound Healing. International Journal of Molecular Sciences. 2023; 24(13):11037. https://doi.org/10.3390/ijms241311037
Chicago/Turabian StyleStoica (Oprea), Alexandra Elena, Delia Albuleț, Alexandra Cătălina Bîrcă, Florin Iordache, Anton Ficai, Alexandru Mihai Grumezescu, Bogdan Ștefan Vasile, Ecaterina Andronescu, Florica Marinescu, and Alina Maria Holban. 2023. "Electrospun Nanofibrous Mesh Based on PVA, Chitosan, and Usnic Acid for Applications in Wound Healing" International Journal of Molecular Sciences 24, no. 13: 11037. https://doi.org/10.3390/ijms241311037