EbbHLH80 Enhances Salt Responses by Up-Regulating Flavonoid Accumulation and Modulating ROS Levels
Abstract
:1. Introduction
2. Results
2.1. Phylogenetic Analysis of EbbHLH80
2.2. Differences in Metabolites with WT and EbbHLH80-OE Seeds
2.3. Transcriptome Profiling of WT and EbbHLH80-OE
2.4. EbbHLH80 Positively Regulates Tobacco Tolerance to Salt Stress
2.5. EbbHLH80 Reduced the Damage Caused by Salt Stress in Transgenic Tobacco Seedlings
2.6. Expression Analysis of Genes Involved in ROS and Flavonoid Biosynthesis
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Metabolite Extraction and Analysis
4.3. RNA Extraction and Sequencing
4.4. RNA Sequencing (RNA-Seq) Differential Gene Expression Analysis
4.5. Determination of H2O2 Content in Seedlings after Salt Stress Treatment
4.6. RT-qPCR Analysis of Gene Expression
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Gao, Q.; Li, X.; Xiang, C.; Li, R.; Xie, H.; Liu, J.; Li, X.; Zhang, G.; Yang, S.; Liang, Y.; et al. EbbHLH80 Enhances Salt Responses by Up-Regulating Flavonoid Accumulation and Modulating ROS Levels. Int. J. Mol. Sci. 2023, 24, 11080. https://doi.org/10.3390/ijms241311080
Gao Q, Li X, Xiang C, Li R, Xie H, Liu J, Li X, Zhang G, Yang S, Liang Y, et al. EbbHLH80 Enhances Salt Responses by Up-Regulating Flavonoid Accumulation and Modulating ROS Levels. International Journal of Molecular Sciences. 2023; 24(13):11080. https://doi.org/10.3390/ijms241311080
Chicago/Turabian StyleGao, Qingqing, Xia Li, Chunfan Xiang, Ruolan Li, Hongchun Xie, Jia Liu, Xiaoning Li, Guanghui Zhang, Shengchao Yang, Yanli Liang, and et al. 2023. "EbbHLH80 Enhances Salt Responses by Up-Regulating Flavonoid Accumulation and Modulating ROS Levels" International Journal of Molecular Sciences 24, no. 13: 11080. https://doi.org/10.3390/ijms241311080