Photomorphogenesis and Photosynthetic Traits Changes in Rice Seedlings Responding to Red and Blue Light
Abstract
:1. Introduction
2. Results
2.1. Plant Height, First Leaf Sheath Length, and Stem Width
2.2. Leaf Growth
2.3. Root Growth
2.4. Root Activity
2.5. Fresh Weight, Dry Weight
2.6. Pigment Content, SPAD
2.7. Chlorophyll Fluorescence
2.8. Photosynthesis
2.9. Rubisco Activity, Gene Expression
2.10. Heat Map Analysis
3. Discussion
3.1. Red and Blue Lights Can Regulate the Early Photomorphogenesis of Rice
3.2. Effects of Red and Blue Lights on Photosynthetic Pigments and Chlorophyll Fluorescence
3.3. Effects of Red and Blue Lights on Photosynthesis, Rubisco Activity, and Gene Expression
4. Materials and Methods
4.1. Plant Materials and a Description of the Experiment
4.2. Morphological Indicators and Root Activity Analysis
4.3. Chlorophyll Content and SPAD of Leaves
4.4. Chlorophyll Fluorescence
4.5. Photosynthetic Parameter Analysis
4.6. Rubisco Activity and Gene Expression
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Variety | Treatments | Leaf Length (cm) | Leaf Width (mm) | Length-Width Ratio | Leaf Area (cm3) | Leaf Number (No. Plant−1) |
---|---|---|---|---|---|---|
XZX24 | CK | 7.26 ± 0.11 c | 3.52 ± 0.03 c | 20.64 ± 0.51 b | 1.4 ± 0.03 b | 2.86 ± 0.04 b |
R | 8.57 ± 0.06 a | 3.11 ± 0.04 d | 27.58 ± 0.40 a | 1.27 ± 0.02 c | 3.34 ± 0.07 a | |
R3B1 | 6.45 ± 0.04 d | 3.42 ± 0.03 c | 18.86 ± 0.30 c | 1.52 ± 0.03 a | 2.90 ± 0.09 b | |
R1B1 | 7.68 ± 0.06 b | 3.85 ± 0.07 b | 19.98 ± 0.25 b | 1.02 ± 0.02 e | 2.98 ± 0.10 b | |
R1B3 | 5.78 ± 0.04 e | 4.05 ± 0.04 a | 14.27 ± 0.19 d | 1.16 ± 0.02 d | 2.90 ± 0.00 b | |
B | 5.44 ± 0.06 f | 4.09 ± 0.04 a | 13.3 ± 0.20 d | 1.01 ± 0.02 e | 3.02 ± 0.08 b | |
HZY261 | CK | 7.01 ± 0.05 b | 3.75 ± 0.04 b | 18.71 ± 0.11 c | 1.26 ± 0.02 ab | 2.74 ± 0.04 c |
R | 8.33 ± 0.04 a | 3.26 ± 0.04 c | 25.53 ± 0.24 a | 1.41 ± 0.02 a | 3.06 ± 0.04 a | |
R3B1 | 6.79 ± 0.14 b | 3.65 ± 0.04 b | 18.62 ± 0.52 c | 1.23 ± 0.12 b | 3.02 ± 0.05 ab | |
R1B1 | 8.02 ± 0.09 a | 3.77 ± 0.10 b | 21.29 ± 0.49 b | 1.32 ± 0.02 ab | 2.90 ± 0.00 b | |
R1B3 | 5.97 ± 0.48 c | 4.15 ± 0.16 a | 14.43 ± 1.20 d | 1.01 ± 0.01 c | 2.74 ± 0.04 c | |
B | 5.53 ± 0.22 c | 4.34 ± 0.03 a | 12.75 ± 0.56 d | 0.95 ± 0.02 c | 3.02 ± 0.08 ab |
Variety | Treatments | Root Length (cm) | Root Surface Area (cm2) | Root Volume (cm3) | Root Diameter (mm) | Root Tip Number (No. Plant−1) |
---|---|---|---|---|---|---|
XZX24 | CK | 62.89 ± 0.28 c | 8.58 ± 0.09 d | 0.28 ± 0.02 b | 1.18 ± 0.01 a | 82.17 ± 0.93 b |
R | 54.80 ± 1.05 d | 7.39 ± 0.07 e | 0.24 ± 0.01 c | 0.96 ± 0.02 b | 69.17 ± 0.60 c | |
R3B1 | 71.55 ± 1.69 a | 11.29 ± 0.17 a | 0.31 ± 0.01 a | 1.24 ± 0.01 a | 96.00 ± 1.04 a | |
R1B1 | 68.29 ± 0.22 b | 9.60 ± 0.20 b | 0.29 ± 0.01 b | 1.22 ± 0.01 a | 93.33 ± 1.42 a | |
R1B3 | 65.79 ± 0.48 b | 9.02 ± 0.10 c | 0.29 ± 0.00 b | 1.21 ± 0.04 a | 93.83 ± 0.60 a | |
B | 49.90 ± 1.00 e | 6.86 ± 0.09 f | 0.19 ± 0.02 d | 0.83 ± 0.01 c | 60.33 ± 1.20 d | |
HZY261 | CK | 42.86 ± 0.26 c | 6.54 ± 0.06 c | 0.21 ± 0.02 c | 0.98 ± 0.04 c | 83.17 ± 2.95 b |
R | 40.44 ± 0.30 d | 6.41 ± 0.20 c | 0.19 ± 0.01 d | 0.85 ± 0.01 d | 66.17 ± 0.67 c | |
R3B1 | 50.15 ± 0.34 a | 13.00 ± 0.31 a | 0.23 ± 0.02 b | 1.22 ± 0.02 b | 91.00 ± 0.58 a | |
R1B1 | 51.19 ± 0.88 a | 13.46 ± 0.30 a | 0.26 ± 0.01 a | 1.32 ± 0.02 a | 92.17 ± 2.03 a | |
R1B3 | 47.38 ± 0.82 b | 8.55 ± 0.16 b | 0.22 ± 0.02 bc | 1.19 ± 0.01 b | 93.17 ± 1.30 a | |
B | 37.85 ± 0.12 e | 5.50 ± 0.09 d | 0.16 ± 0.00 e | 0.78 ± 0.01 d | 50.83 ± 1.42 d |
Gene | Accession No. | Up-Primer (5′-3′) | Down-Primer (5′-3′) |
---|---|---|---|
OsActin3 | Os03g0718100 | CCACTATGTTCCCTGGCATT | GTACTCAGCCTTGGCAATCC |
OsRBCS2 | AK121444 | CAGCAAGGTCGGATTCGTCT | CACACGAAACAAGGTGGGAG |
OsRBCS3 | AK068555 | TTCCAAGGGCACAAGTCCAC | TAGGCAGCAATCCACCAGC |
OsRBCS4 | AK068266 | GTGGATGAGTAAAGGGACAA | CAGGAGTAAATCAAGAGCGT |
OsRBCS5 | AK099574 | TACCTGCTCCGGTCCAAATG | TCAAAGCTGCCAAAACCGAA |
OsRBCSL | OrsajCp033 | CCCCGGAGTACGAAACCAAG | GAGGCGGACCTTGGAAAGTT |
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Ren, M.; Liu, S.; Tang, C.; Mao, G.; Gai, P.; Guo, X.; Zheng, H.; Tang, Q. Photomorphogenesis and Photosynthetic Traits Changes in Rice Seedlings Responding to Red and Blue Light. Int. J. Mol. Sci. 2023, 24, 11333. https://doi.org/10.3390/ijms241411333
Ren M, Liu S, Tang C, Mao G, Gai P, Guo X, Zheng H, Tang Q. Photomorphogenesis and Photosynthetic Traits Changes in Rice Seedlings Responding to Red and Blue Light. International Journal of Molecular Sciences. 2023; 24(14):11333. https://doi.org/10.3390/ijms241411333
Chicago/Turabian StyleRen, Maofei, Shanzhen Liu, Chengzhu Tang, Guiling Mao, Panpan Gai, Xiaoli Guo, Huabin Zheng, and Qiyuan Tang. 2023. "Photomorphogenesis and Photosynthetic Traits Changes in Rice Seedlings Responding to Red and Blue Light" International Journal of Molecular Sciences 24, no. 14: 11333. https://doi.org/10.3390/ijms241411333