A 43 Bp-Deletion in the F3′H Gene Reducing Anthocyanins Is Responsible for Keeping Buds Green at Low Temperatures in Broccoli
Abstract
:1. Introduction
2. Results
2.1. Anthocyanin Contents
2.2. Mapping of the Green/Purple Bud Trait at Cold Temperatures in Broccoli
2.3. Fine Mapping
2.4. The Application of the KASP Markers
2.5. Expression Profile of Anthocyanin-Related Genes in the Mutant and the Wild Line
2.6. Validation of Anthocyanin-Related Genes by qRT-PCR
2.7. cDNA and Predicted Amino Acid Sequences of the F3′H Gene in HX-11 and HX-16
3. Discussion
4. Materials and Methods
4.1. Construction of F2 and Backcross Populations
4.2. Anthocyanin Extraction and Measurement
4.3. Genomic DNA Extraction and Library Construction
4.4. QTL Mapping
4.5. KASP Primer Designing and Genotyping
4.6. Transcriptome Analysis
4.7. The CDS and Predicted Amino Acid Sequences of F3′H in HX-11 and HX-16
4.8. qRT-PCR Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Total Anthocyanins C µg/g | CC µg/g | DC µg/g | MvC µg/g | PelC µg/g | PeoC µg/g | PtC µg/g | |
---|---|---|---|---|---|---|---|
GS | 12.123 ± 0.770 c | 3.830 ± 0.420 b | 4.190 ± 0.210 b | 0.002 ± 0.011 b | 0 | 0 | 0 |
PS | 20.712 ± 0.378 b | 6.980 ± 0.280 b | 5.690 ± 0.090 b | 0.002 ± 0.012 b | 0 | 0 | 0 |
PB | 103.138 ± 4.399 a | 87.890 ± 4.480 a | 9.110 ± 1.360 a | 0.881 ± 0.323 a | 0 | 0 | 0 |
Material Codes | Species | Material Codes | Species | Material Codes | Species |
---|---|---|---|---|---|
1015 | B. oleracea L. var. italica | SG1921 | B. oleracea L. var. italica | SG1911 | B. oleracea L. var. italica |
1403 | B. oleracea L. var. italica | SG1614 | B. oleracea L. var. botrytis | SG1912-1 | B. oleracea L. var. italica |
1405-1 | B. oleracea L. var. italica | 51932-2 | B. oleracea L. var. italica | SG1920 | B. oleracea L. var. italica |
1409 | B. oleracea L. var. italica | 51936-1 | B. oleracea L. var. italica | SG2009-2 | Brassica oleracea L. var. kohlrabi |
1502-1 | B. oleracea L. var. italica | 51938-13 | B. oleracea L. var. italica | SG2021 | B. oleracea L. var. acephala |
1610-4B | B. oleracea L. var. italica | PR2003 | B. oleracea L. var. botrytis | UC001 | B. oleracea L. var. italica |
1910-1 | B. oleracea L. var. italica | 2035 | B. oleracea L. var. italica | UC002 | B. oleracea L. var. botrytis |
2035 | B. oleracea L. var. italica | 1947 | B. oleracea L. var. italica | UC003 | B. oleracea L. var. capitata |
2057-11 | B. oleracea L. var. italica | VR2003 | B. oleracea L. var. botrytis | UC012 | B. oleracea L. var. gongylodes |
5815-6A | B. oleracea L. var. italica | SG1401-1 | B. oleracea L. var. italica | UC018 | B. oleracea L. var. gemmifera |
K2145 | B. oleracea L. var. acephala | SG1913 | B. oleracea L. var. italica | UC044 | B. oleracea L. var. italica |
K2150 | B. oleracea L. var. caulorapa | SG1914-1 | B. oleracea L. var. italica | UC019 | B. oleracea L. var. acephala |
2018K1M3 | B. oleracea L. var. acephala | SG1917-2 | B. oleracea L. var. italica | UC020 | B. oleracea L. var. gongylodes |
2018K1M5 | B. oleracea L. var. acephala | SG1908 | B. oleracea L. var. italica | UC030 | B. oleracea L. var. botrytis |
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Yu, H.; Wang, J.; Shen, Y.; Sheng, X.; Shaw, R.K.; Branca, F.; Gu, H. A 43 Bp-Deletion in the F3′H Gene Reducing Anthocyanins Is Responsible for Keeping Buds Green at Low Temperatures in Broccoli. Int. J. Mol. Sci. 2023, 24, 11391. https://doi.org/10.3390/ijms241411391
Yu H, Wang J, Shen Y, Sheng X, Shaw RK, Branca F, Gu H. A 43 Bp-Deletion in the F3′H Gene Reducing Anthocyanins Is Responsible for Keeping Buds Green at Low Temperatures in Broccoli. International Journal of Molecular Sciences. 2023; 24(14):11391. https://doi.org/10.3390/ijms241411391
Chicago/Turabian StyleYu, Huifang, Jiansheng Wang, Yusen Shen, Xiaoguang Sheng, Ranjan Kumar Shaw, Ferdinando Branca, and Honghui Gu. 2023. "A 43 Bp-Deletion in the F3′H Gene Reducing Anthocyanins Is Responsible for Keeping Buds Green at Low Temperatures in Broccoli" International Journal of Molecular Sciences 24, no. 14: 11391. https://doi.org/10.3390/ijms241411391