Identification of the Intestinal Microbes Associated with Locomotion
Abstract
:1. Introduction
2. Results
2.1. A Random Colonization of Human Gut Microbiome into Conventional Mice through FMT Had a Different Impact on Locomotion
2.2. Different Types of Gut Microbiome Were Established in Each of the Experimental Mice after FMT with Human Feces
2.3. The Differences in Locomotion Were Correlated with the Shifts in the Composition of the Gut Microbiome
2.4. Different Microbial Communities Were Established in Each of the Three Groups of Mice following the FMT
2.5. Intestinal Microbes That Affect Locomotion Were Identified at the Species Level
3. Discussion
4. Materials and Methods
4.1. Study Design and Animal Experiment
4.2. Wire Hanging Test
4.3. Analyses of Biochemical Parameters
4.4. Histological Analysis
4.5. DNA Extraction and 16S rRNA Gene Sequencing
4.6. Data and Statistical Analyses
4.7. The α-Diversity Analysis for Relative Abundance Evaluation of Material and Microbiome
4.8. The β-Analysis for Relative Abundance Evaluation of Material and Microbiome
4.9. Construction of Heatmap and Phylogenetic Tree
4.10. Co-Occurrence Network Construction
4.11. Differential Abundance
4.12. Quantification and Statistical Analysis
4.13. Ethics Approval
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ahn, J.-S.; Choi, Y.-J.; Kim, H.-B.; Chung, H.-J.; Hong, S.-T. Identification of the Intestinal Microbes Associated with Locomotion. Int. J. Mol. Sci. 2023, 24, 11392. https://doi.org/10.3390/ijms241411392
Ahn J-S, Choi Y-J, Kim H-B, Chung H-J, Hong S-T. Identification of the Intestinal Microbes Associated with Locomotion. International Journal of Molecular Sciences. 2023; 24(14):11392. https://doi.org/10.3390/ijms241411392
Chicago/Turabian StyleAhn, Ji-Seon, Yu-Jin Choi, Han-Byeol Kim, Hea-Jong Chung, and Seong-Tshool Hong. 2023. "Identification of the Intestinal Microbes Associated with Locomotion" International Journal of Molecular Sciences 24, no. 14: 11392. https://doi.org/10.3390/ijms241411392