Effective Healing of Staphylococcus aureus-Infected Wounds in Pig Cathelicidin Protegrin-1-Overexpressing Transgenic Mice
Abstract
:1. Introduction
2. Results and Discussion
2.1. Enhanced Wound Healing in Staphylococcus aureus-Infected Wounds in PG1 Tg Mice
2.2. Bacterial Clearance in Infected Wounds of PG1 Tg Mice
2.3. Efficient Formation of Neo-epithelium in PG1 Tg Mice with Infected Wounds
2.4. Successful Production of Recombinant PG1 Using a Bacterial Expression System
2.5. Enhancement of Epidermal Keratinocyte Migration by PG1
2.6. Immunomodulatory Effects of PG1 on Epidermal KCs
3. Materials and Methods
3.1. Animal Experiments
3.2. Determination of Bacteria Load in Wound Biopsies
3.3. Histological Analyses
3.4. KC Isolation
3.5. Production of Recombinant PG1 Peptides
3.6. Determination of PG1 Antimicrobial Activity
3.7. Cell Scratch Assay
3.8. Migration Assay
3.9. Real-Time Quantitative PCR
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Experimental Conditions | Day 0 (CFUs/g) | Day 3 (CFUs/g) | Day 7 (CFUs/g) | Day 14 (CFUs/g) |
---|---|---|---|---|
PG1 mice with uninfected wounds (group 1) 1 | 0 | 0 | 3 ± 4.3 | 5 ± 5.1 |
Wild-type mice with S. aureus-infected wounds (group 2) 1 | 1.85 ± 0.8 × 106 * | 8.21 ± 3.4 × 108 | 8.75 ± 3.2 × 108 | Fatal |
Wild-type mice with S. aureus-infected wounds treated with gentamycin for 3 days (group 3) 1 | 1.85 ± 0.8 × 106 * | 4.89 ± 2.3 × 105 | 1.41 ± 0.9 × 102 | 41 ± 3.8 |
PG1 transgenic mouse with S. aureus-infected wounds that were not subjected to antibiotic treatment (group 4) 1 | 1.85 ± 0.8 × 106 * | 6.5 ± 2.7 × 104 | 1.39 ± 2.4 × 102 | 31 ± 4.2 |
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Soundrarajan, N.; Somasundaram, P.; Kim, D.; Cho, H.-S.; Jeon, H.; Ahn, B.; Kang, M.; Song, H.; Park, C. Effective Healing of Staphylococcus aureus-Infected Wounds in Pig Cathelicidin Protegrin-1-Overexpressing Transgenic Mice. Int. J. Mol. Sci. 2023, 24, 11658. https://doi.org/10.3390/ijms241411658
Soundrarajan N, Somasundaram P, Kim D, Cho H-S, Jeon H, Ahn B, Kang M, Song H, Park C. Effective Healing of Staphylococcus aureus-Infected Wounds in Pig Cathelicidin Protegrin-1-Overexpressing Transgenic Mice. International Journal of Molecular Sciences. 2023; 24(14):11658. https://doi.org/10.3390/ijms241411658
Chicago/Turabian StyleSoundrarajan, Nagasundarapandian, Prathap Somasundaram, Dohun Kim, Hye-Sun Cho, Hyoim Jeon, Byeonyong Ahn, Mingue Kang, Hyuk Song, and Chankyu Park. 2023. "Effective Healing of Staphylococcus aureus-Infected Wounds in Pig Cathelicidin Protegrin-1-Overexpressing Transgenic Mice" International Journal of Molecular Sciences 24, no. 14: 11658. https://doi.org/10.3390/ijms241411658
APA StyleSoundrarajan, N., Somasundaram, P., Kim, D., Cho, H. -S., Jeon, H., Ahn, B., Kang, M., Song, H., & Park, C. (2023). Effective Healing of Staphylococcus aureus-Infected Wounds in Pig Cathelicidin Protegrin-1-Overexpressing Transgenic Mice. International Journal of Molecular Sciences, 24(14), 11658. https://doi.org/10.3390/ijms241411658