Enhanced Cell Wall and Cell Membrane Activity Promotes Heat Adaptation of Enterococcus faecium

Wang, Li; Li, Aike; Fang, Jun; Wang, Yongwei; Chen, Lixian; Qiao, Lin; Wang, Weiwei

doi:10.3390/ijms241411822

Open AccessArticle

Enhanced Cell Wall and Cell Membrane Activity Promotes Heat Adaptation of Enterococcus faecium

by

Li Wang

^1,2,

Aike Li

¹,

Jun Fang

²,

Yongwei Wang

¹,

Lixian Chen

¹,

Lin Qiao

^1,* and

Weiwei Wang

^1,*

¹

Academy of National Food and Strategic Reserves Administration, Beijing 100037, China

²

College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China

^*

Authors to whom correspondence should be addressed.

Int. J. Mol. Sci. 2023, 24(14), 11822; https://doi.org/10.3390/ijms241411822

Submission received: 14 June 2023 / Revised: 9 July 2023 / Accepted: 21 July 2023 / Published: 23 July 2023

(This article belongs to the Special Issue Multi-Omics Approaches for Health and Disease)

Download

Browse Figures

Review Reports Versions Notes

Abstract

Enterococcus faecium (E. faecium) is widely used in foods and is known as a probiotic to treat or prevent diarrhea in pets and livestock. However, the poor resistance of E. faecium to high temperature processing procedures limits its use. Strain domestication is a low-cost and effective method to obtain high-temperature-resistant strains. In this study, heat treatment was performed from 45 °C to 70 °C and the temperature was gradually increased by 5 °C every 3 days. After domestication, the survival rates of the high temperature adaptation strain RS047-wl under 65 °C water bath for 40 min was 11.5 times higher than WT RS047. Moreover, the saturated fatty acid (SFA) contents in cell membrane and the cell volume significantly increased in the RS047-wl. The combined transcriptomic, metabolomic, and proteomics analysis results showed a significant enhancement of cell wall and membrane synthesis ability in the RS047-wl. In conclusion, one of the main factors contributing to the improved high temperature resistance of RS047-wl was its enhanced ability to synthesize cell wall and membrane, which helped maintain normal cell morphology. Developing a high-temperature-resistant strain and understanding its mechanism enables it to adapt to high temperatures. This lays the groundwork for its future development and application.

Keywords: Enterococcus faecium; heat adaptation; multi-omics; cell wall; cell membrane

Share and Cite

MDPI and ACS Style

Wang, L.; Li, A.; Fang, J.; Wang, Y.; Chen, L.; Qiao, L.; Wang, W. Enhanced Cell Wall and Cell Membrane Activity Promotes Heat Adaptation of Enterococcus faecium. Int. J. Mol. Sci. 2023, 24, 11822. https://doi.org/10.3390/ijms241411822

AMA Style

Wang L, Li A, Fang J, Wang Y, Chen L, Qiao L, Wang W. Enhanced Cell Wall and Cell Membrane Activity Promotes Heat Adaptation of Enterococcus faecium. International Journal of Molecular Sciences. 2023; 24(14):11822. https://doi.org/10.3390/ijms241411822

Chicago/Turabian Style

Wang, Li, Aike Li, Jun Fang, Yongwei Wang, Lixian Chen, Lin Qiao, and Weiwei Wang. 2023. "Enhanced Cell Wall and Cell Membrane Activity Promotes Heat Adaptation of Enterococcus faecium" International Journal of Molecular Sciences 24, no. 14: 11822. https://doi.org/10.3390/ijms241411822

APA Style

Wang, L., Li, A., Fang, J., Wang, Y., Chen, L., Qiao, L., & Wang, W. (2023). Enhanced Cell Wall and Cell Membrane Activity Promotes Heat Adaptation of Enterococcus faecium. International Journal of Molecular Sciences, 24(14), 11822. https://doi.org/10.3390/ijms241411822

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Enhanced Cell Wall and Cell Membrane Activity Promotes Heat Adaptation of Enterococcus faecium

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI