Function of the R2R3-MYB Transcription Factors in Dalbergia odorifera and Their Relationship with Heartwood Formation
Abstract
:1. Introduction
2. Results
2.1. Sequence Characterization and Physicochemical Properties of D. odorifera R2R3-MYB Transcription Factors
2.2. Gene Structure and Motif Analysis of D. odorifera R2R3-MYB Transcription Factor Family
2.3. Chromosomal Localization and Synteny Analysis of D. odorifera R2R3-MYB TFs
2.4. Evolutionary Relationship Analysis of D. odorifera and A. thaliana R2R3-MYB TFs
2.5. Expression Patterns of D. odorifera R2R3-MYB Genes in Different Tissues
2.6. Subcellular Location of DodMYB89
2.7. Validation of Transcriptional Activation Activity of DodMYB89
2.8. DodMYB89 Affects the Biosynthesis of Flavonoid Components
3. Discussion
4. Materials and Methods
4.1. Screening of the R2R3-MYB Transcription Factor Family in the D. odorifera Genome
4.2. Conserved Motif of Protein and Gene Structure Analysis of R2R3-MYB TFs
4.3. Chromosome Localization and Covariance Analysis
4.4. Phylogenetic Analysis
4.5. Plant Material and Sample Preparation
4.6. RNA Extraction and Transcriptome Sequencing
4.7. Subcellular Localization
4.8. Transcriptional Activation Activity of DodMYB89
4.9. Transient Dual-Luciferase Assays
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ma, R.; Luo, J.; Wang, W.; Song, T.; Fu, Y. Function of the R2R3-MYB Transcription Factors in Dalbergia odorifera and Their Relationship with Heartwood Formation. Int. J. Mol. Sci. 2023, 24, 12430. https://doi.org/10.3390/ijms241512430
Ma R, Luo J, Wang W, Song T, Fu Y. Function of the R2R3-MYB Transcription Factors in Dalbergia odorifera and Their Relationship with Heartwood Formation. International Journal of Molecular Sciences. 2023; 24(15):12430. https://doi.org/10.3390/ijms241512430
Chicago/Turabian StyleMa, Ruoke, Jia Luo, Weijie Wang, Tianqi Song, and Yunlin Fu. 2023. "Function of the R2R3-MYB Transcription Factors in Dalbergia odorifera and Their Relationship with Heartwood Formation" International Journal of Molecular Sciences 24, no. 15: 12430. https://doi.org/10.3390/ijms241512430