Endocytosis and Endocytic Motifs across the Connexin Gene Family
Abstract
:1. Introduction
2. Results
2.1. Connexin Family Members Contain Both Canonical Tyrosine- and Di-Leucine-Based Endocytic Motifs
2.2. Non-Canonical Tyrosine and Di-Leucine-Based Endocytic Motifs in Connexins
2.3. Location of Endocytic Motifs in Connexins
2.4. Conservation of Endocytic Motifs
2.5. Phylogenetic Relation of Endocytic Motifs
2.6. Conserved Canonical and Non-Canonical Di-Leucine-Type Motifs Located in the TM4/CT Transition Zone
2.7. Connexins with and without Identifiable Endocytic Motifs Endocytose to Form Typical Cytoplasmic Annular Gap Junctions
3. Discussion
4. Materials and Methods
4.1. Endocytic Motif Search
4.2. Live Cell Imaging
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Cx Gene Name | Cx Protein Name | Type of Endocytic Signal | Location of Endocytic Signal | |
---|---|---|---|---|
Intracellular-Loop | C-Terminal Domain | |||
A1 | CX43 * | Tyrosine based (YXXF) | --- | Y230VFF; Y265AYF; Y286KLV |
A4 | CX37 * | --- | Y266LPV; Y281NGL | |
A8 | CX50 | --- | Y266QLL; Y278FPL; (Y316QETL); Y323AQV | |
A9 | CX59 | --- | (D283YNLL); Y284NLL; Y297PSL; (L500TNNLI) | |
A10 | CX62 | --- | (L433SRLL); Y513VCV | |
B4 | CX30.3 | Y117DNL | --- | |
C1 | CX45 * | Y141PEM | Y301TEL | |
C2 | CX47 | --- | Y330SLV | |
D2 | CX36 * | Y109STV | --- | |
E1 | CX23 | --- | Y200FPF | |
B1 | CX32 * | Di-leucine based | (E103KKMLR) | E247INKLL; (L260KDILR) |
B6 | CX30 | --- | E243MNELI | |
C3 | CX30.2 (CX31.3) | E111EETLI | --- | |
D3 | CX31.9 | --- | E233AQKLL | |
A3 | CX46 * | Non-canonical or Not identifiable | --- | (D296FKLL); (E367AGAAPLL) |
A5 | CX40 | (E105KRKLR) | (L145QGTLL) | |
B2 | CX26 * | --- | --- | |
B3 | CX31 | --- | --- | |
B5 | CX31.1 | --- | (L245SGDLI); (D255SHPPLL) | |
B7 | CX25 | --- | --- | |
D4 | CX40.1 | --- | --- |
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Fisher, C.G.; Falk, M.M. Endocytosis and Endocytic Motifs across the Connexin Gene Family. Int. J. Mol. Sci. 2023, 24, 12851. https://doi.org/10.3390/ijms241612851
Fisher CG, Falk MM. Endocytosis and Endocytic Motifs across the Connexin Gene Family. International Journal of Molecular Sciences. 2023; 24(16):12851. https://doi.org/10.3390/ijms241612851
Chicago/Turabian StyleFisher, Charles G., and Matthias M. Falk. 2023. "Endocytosis and Endocytic Motifs across the Connexin Gene Family" International Journal of Molecular Sciences 24, no. 16: 12851. https://doi.org/10.3390/ijms241612851