Molecular Relay Stations in Membrane Nanotubes: IRSp53 Involved in Actin-Based Force Generation
Abstract
:1. Introduction
2. Results
2.1. IRSp53 Is Expressed in the NTs of COS-7 Kidney and A20 B Lymphoma Cells
2.2. Overexpression of IRSp53 Protein and Its I-BAR Domain in COS-7 Kidney Cells
Parameter | COS-7 Control | I-BAR Overexpression | IRSp53 Overexpression |
---|---|---|---|
Number of filopodia per cell | 2 | 62 ± 4 | 38 ± 3 |
Average length of filopodia (µm) | 1.80 ± 0.13 | 3.90 ± 0.19 | 8.15 ± 0.33 |
Relative frequency of NTs | 0.29 ± 0.02 | 0.75 ± 0.03 | 0.54 ± 0.03 |
Average diameter of the NTs (µm) | 1.06 ± 0.03 | 0.56 ± 0.01 | 0.67 ± 0.02 |
Length of the NTs (µm) | 21.50 ± 1.13 | 20.25 ± 0.77 | 36.13 ± 1.55 |
Relative branching | 0.33 ± 0.11 | 0.77 ± 0.12 | 0.78 ± 0.06 |
Length of the branches (µm) | 4.80 ± 0.42 | 5.82 ± 0.34 | 8.77 ± 0.50 |
Accumulation level in the NTs | 0.22 ± 0.05 | 0.50 ± 0.07 | 0.50 ± 0.08 |
2.3. Overexpression of IRSp53 Protein and Its I-BAR Domain in A20 B Lymphoma Cells
2.4. The Effects of IRSp53 and Its I-BAR Domain on Actin Polymerisation
3. Discussion
3.1. Background and Aim
3.2. Key Observations
3.3. General Concept
3.4. The Role of the IRSp53
3.5. Cell Line Differences
3.6. Model
4. Materials and Methods
4.1. Protein Purification and Labelling
4.2. Cells, Culture, Cell Transfection
4.3. Immunocytochemistry and Cell Staining
4.4. Microscopic Imaging
4.5. Statistical Analysis
4.6. Total Internal Reflection Fluorescence Microscopy
4.7. Pyrenyl Polymerisation Assay
4.8. General Experimental Conditions
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | A20 Control | I-BAR Overexpression | IRSp53 Overexpression |
---|---|---|---|
Number of filopodia per cell | 4.33 ± 0.51 | 16.30 ± 2.12 | 13.86 ± 1.65 |
Average length of filopodia (µm) | 1.24 ± 0.06 | 3.05 ± 0.16 | 2.18 ± 0.11 |
Relative frequency of NTs | 0.16 ± 0.03 | 0.12 ± 0.03 | 0.19 ± 0.04 |
Average diameter of the NTs (µm) | 1.35 ± 0.10 | 0.93 ± 0.12 | 1.24 ±0.08 |
Length of the NTs (µm) | 26.33 ± 5.59 | 21.36 ± 2.29 | 31.75 ± 4.17 |
Relative branching | 0.12 ± 0.80 | 0.55 ± 0.19 | 1.00 ± 0.31 |
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Madarász, T.; Brunner, B.; Halász, H.; Telek, E.; Matkó, J.; Nyitrai, M.; Szabó-Meleg, E. Molecular Relay Stations in Membrane Nanotubes: IRSp53 Involved in Actin-Based Force Generation. Int. J. Mol. Sci. 2023, 24, 13112. https://doi.org/10.3390/ijms241713112
Madarász T, Brunner B, Halász H, Telek E, Matkó J, Nyitrai M, Szabó-Meleg E. Molecular Relay Stations in Membrane Nanotubes: IRSp53 Involved in Actin-Based Force Generation. International Journal of Molecular Sciences. 2023; 24(17):13112. https://doi.org/10.3390/ijms241713112
Chicago/Turabian StyleMadarász, Tamás, Brigitta Brunner, Henriett Halász, Elek Telek, János Matkó, Miklós Nyitrai, and Edina Szabó-Meleg. 2023. "Molecular Relay Stations in Membrane Nanotubes: IRSp53 Involved in Actin-Based Force Generation" International Journal of Molecular Sciences 24, no. 17: 13112. https://doi.org/10.3390/ijms241713112