The Pleiotropic Effects of YBX1 on HTLV-1 Transcription
Abstract
:1. Introduction
2. Results
2.1. HBZ Interacts with the Cellular Factor YBX1
2.2. YBX1 Activates Viral Transcription
2.3. YBX1 Interacts with Tax
2.4. YBX1 Activates Sense and Antisense Viral Transcription
2.5. YBX1 Associates with the 5′ and 3′ Viral LTR
2.6. YBX1 Enhances Tax Binding and HBZ Represses YBX1 Transcriptional Activation
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Plasmids
4.3. S-Tag-Affinity Pulldown Assay
4.4. Mass Spectrometry and Proteomic Analysis
4.5. Immunoprecipitation
4.6. Transfections, Luciferase Reporter Assays, and p19 Gag ELISA
4.7. Immunoblotting
4.8. Lentiviral Production and Cell Transduction
4.9. Chromatin Immunoprecipitation (ChIP)
4.10. Quantitative RT-PCR (qRT-PCR)
4.11. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Deletion Mutant | Primer Sequence |
---|---|
∆A/P (∆1–55) | Forward primer: 5′-GCAACGAAGGTTTTGGGAACAGTAAAATG-3′ Reverse primer: 5′-CAAAACCTTCGTTGCGGTTCCCATGGTGG-3′ |
∆CSD (∆56–128) | Forward primer: 5′-GGTGGTGTTCCAGTTCAAGGCAGTAAATATG-3′ Reverse primer: 5′-GAACTGGAACACCACCGATGACCTTCTTGTC-3′ |
∆CTD (∆129–324) | Forward primer: 5′-CAAATGTTACAGGTCCTTAAGCGGCCGCACTC-3′ Reverse primer: 5′-GGACCTGTAACATTTGCTGCCTCCGCAC-3′ |
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Smith, S.; Seth, J.; Midkiff, A.; Stahl, R.; Syu, Y.-C.; Shkriabai, N.; Kvaratskhelia, M.; Musier-Forsyth, K.; Jain, P.; Green, P.L.; et al. The Pleiotropic Effects of YBX1 on HTLV-1 Transcription. Int. J. Mol. Sci. 2023, 24, 13119. https://doi.org/10.3390/ijms241713119
Smith S, Seth J, Midkiff A, Stahl R, Syu Y-C, Shkriabai N, Kvaratskhelia M, Musier-Forsyth K, Jain P, Green PL, et al. The Pleiotropic Effects of YBX1 on HTLV-1 Transcription. International Journal of Molecular Sciences. 2023; 24(17):13119. https://doi.org/10.3390/ijms241713119
Chicago/Turabian StyleSmith, Susan, Jaideep Seth, Amanda Midkiff, Rachel Stahl, Yu-Ci Syu, Nikoloz Shkriabai, Mamuka Kvaratskhelia, Karin Musier-Forsyth, Pooja Jain, Patrick L. Green, and et al. 2023. "The Pleiotropic Effects of YBX1 on HTLV-1 Transcription" International Journal of Molecular Sciences 24, no. 17: 13119. https://doi.org/10.3390/ijms241713119