Regulation of Glycoprotein VI-Dependent Platelet Activation and Thrombus Formation by Heparan Sulfate Proteoglycan Perlecan
Abstract
:1. Introduction
2. Results
2.1. Perlecan Enhances GPVI-Induced Platelet Activation
2.2. Perlecan Induces Platelet Spreading
2.3. Dual Modulating Effect of Perlecan in GPVI-Dependent Thrombus Formation
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Blood Donors and Platelet Isolation
4.3. Flow Cytometry
4.4. Platelet Spreading
4.5. Microfluidic Thrombus Formation
4.6. Assessment of Thrombus Parameters
4.7. Culturing of Endothelial Cells
4.8. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Provenzale, I.; De Simone, I.; Gibbins, J.M.; Heemskerk, J.W.M.; van der Meijden, P.E.J.; Jones, C.I. Regulation of Glycoprotein VI-Dependent Platelet Activation and Thrombus Formation by Heparan Sulfate Proteoglycan Perlecan. Int. J. Mol. Sci. 2023, 24, 13352. https://doi.org/10.3390/ijms241713352
Provenzale I, De Simone I, Gibbins JM, Heemskerk JWM, van der Meijden PEJ, Jones CI. Regulation of Glycoprotein VI-Dependent Platelet Activation and Thrombus Formation by Heparan Sulfate Proteoglycan Perlecan. International Journal of Molecular Sciences. 2023; 24(17):13352. https://doi.org/10.3390/ijms241713352
Chicago/Turabian StyleProvenzale, Isabella, Ilaria De Simone, Jonathan M. Gibbins, Johan W. M. Heemskerk, Paola E. J. van der Meijden, and Chris I. Jones. 2023. "Regulation of Glycoprotein VI-Dependent Platelet Activation and Thrombus Formation by Heparan Sulfate Proteoglycan Perlecan" International Journal of Molecular Sciences 24, no. 17: 13352. https://doi.org/10.3390/ijms241713352