A Strainer-Based Platform for the Collection and Immunolabeling of Mouse Intestinal Organoids

Tan, Jinlong; Liu, Yinju; Li, Weike; Chen, Guohua; Fang, Yongxiang; He, Xiaobing; Fu, Baoquan; Jing, Zhizhong

doi:10.3390/ijms241713568

Open AccessArticle

A Strainer-Based Platform for the Collection and Immunolabeling of Mouse Intestinal Organoids

by

Jinlong Tan

^†

,

Yinju Liu

^†,

Weike Li

,

Guohua Chen

,

Yongxiang Fang

,

Xiaobing He

,

Baoquan Fu

^* and

Zhizhong Jing

^*

State Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Public Health of Agriculture Ministry Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2023, 24(17), 13568; https://doi.org/10.3390/ijms241713568

Submission received: 11 August 2023 / Revised: 29 August 2023 / Accepted: 31 August 2023 / Published: 1 September 2023

(This article belongs to the Special Issue Organoid Culture and Characterization Systems for Tissue Engineering Applications)

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Abstract

Intestinal organoids have emerged as powerful model systems for studying the complex structure and function of the intestine. However, there is a lack of widely applicable methods for the collection, labeling, and imaging of intestinal organoids. In this study, we developed a novel method for loading and labeling intestinal organoids, a method that efficiently collects the organoids and facilitates imaging of their three-dimensional (3D) structure. Based on this strainer platform, mouse intestinal organoids were adequately collected and immobilized, facilitating the immunolabeling workflow to target proteins of the organoids. After evaluation, the strainer size of 40 μm was considered to be more conducive to the collection and labeling of mouse intestinal organoids. More extensive research on organoids of multiple types and species origins will contribute to broadening the applicability of the methodology. Overall, our study proposes an innovative workflow for loading and analyzing intestinal organoids. The combination of a strainer-based collection method, fluorescent labeling, and 3D reconstruction provides valuable insights into the organization and complexity of these tissue models, thereby offering new avenues for investigating intestinal development, disease modeling, and drug discovery.

Keywords: organoid; collection; labeling; 3D imaging; strainer platform

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MDPI and ACS Style

Tan, J.; Liu, Y.; Li, W.; Chen, G.; Fang, Y.; He, X.; Fu, B.; Jing, Z. A Strainer-Based Platform for the Collection and Immunolabeling of Mouse Intestinal Organoids. Int. J. Mol. Sci. 2023, 24, 13568. https://doi.org/10.3390/ijms241713568

AMA Style

Tan J, Liu Y, Li W, Chen G, Fang Y, He X, Fu B, Jing Z. A Strainer-Based Platform for the Collection and Immunolabeling of Mouse Intestinal Organoids. International Journal of Molecular Sciences. 2023; 24(17):13568. https://doi.org/10.3390/ijms241713568

Chicago/Turabian Style

Tan, Jinlong, Yinju Liu, Weike Li, Guohua Chen, Yongxiang Fang, Xiaobing He, Baoquan Fu, and Zhizhong Jing. 2023. "A Strainer-Based Platform for the Collection and Immunolabeling of Mouse Intestinal Organoids" International Journal of Molecular Sciences 24, no. 17: 13568. https://doi.org/10.3390/ijms241713568

APA Style

Tan, J., Liu, Y., Li, W., Chen, G., Fang, Y., He, X., Fu, B., & Jing, Z. (2023). A Strainer-Based Platform for the Collection and Immunolabeling of Mouse Intestinal Organoids. International Journal of Molecular Sciences, 24(17), 13568. https://doi.org/10.3390/ijms241713568

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A Strainer-Based Platform for the Collection and Immunolabeling of Mouse Intestinal Organoids

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