Physiological, Proteomic, and Resin Yield-Related Genes Expression Analysis Provides Insights into the Mechanisms Regulating Resin Yield in Masson Pine
Abstract
:1. Introduction
2. Results
2.1. Physiological Characteristics of High and Low-Resin-Yielding Masson Pines
2.2. Proteome Profiling and DEPs Identification
2.3. Function Classification and Metabolic Pathways Enrichment Analysis of DEPs
2.4. DEPs Expression Trend Analysis using Mfuzz Clustering
2.5. Identification of DEPS Involved in Resin Yield
2.6. Change Patterns of Resin Yield-Related Genes between High and Low Resin Resin-Yielding Masson Pines
2.7. Parallel Reaction Monitoring Validation
3. Discussion
3.1. Carbohydrate Metabolism and Terpenoid Biosynthesis Regulate Resin Yield via Promoting Resin Biosynthesis
3.2. ABC and GST Improve Resin Yield via Facilitating Resin Transport
3.3. Stress-Related Proteins Involve in Resin Yield via Regulating Responses to Stress
3.4. Plant Hormones Highly Regulate Resin Yield via Modulating Terpenoid Biosynthesis as Well as Xylem Growth and Differentiation
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Determination of Terpene Synthase Activity
4.3. Measurement of Soluble Sugar and Sucrose Contents
4.4. Quantification of Hormone Contents
4.5. Protein Extraction and Trypsin Digestion
4.6. TMT Labeling and LC-MS/MS Analysis
4.7. Protein Identification and Quantitation
4.8. Bioinformatics Analysis
4.9. Gene Expression Analysis
4.10. Parallel Reaction Monitoring Analysis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Li, Z.; Zhou, Z.; Hou, Q.; Shen, L.; Zhao, H.; Wen, X. Physiological, Proteomic, and Resin Yield-Related Genes Expression Analysis Provides Insights into the Mechanisms Regulating Resin Yield in Masson Pine. Int. J. Mol. Sci. 2023, 24, 13813. https://doi.org/10.3390/ijms241813813
Li Z, Zhou Z, Hou Q, Shen L, Zhao H, Wen X. Physiological, Proteomic, and Resin Yield-Related Genes Expression Analysis Provides Insights into the Mechanisms Regulating Resin Yield in Masson Pine. International Journal of Molecular Sciences. 2023; 24(18):13813. https://doi.org/10.3390/ijms241813813
Chicago/Turabian StyleLi, Zhengchun, Zijing Zhou, Qiandong Hou, Luonan Shen, Hong Zhao, and Xiaopeng Wen. 2023. "Physiological, Proteomic, and Resin Yield-Related Genes Expression Analysis Provides Insights into the Mechanisms Regulating Resin Yield in Masson Pine" International Journal of Molecular Sciences 24, no. 18: 13813. https://doi.org/10.3390/ijms241813813