HCN2 Channels in the Ventral Hippocampal CA1 Regulate Nociceptive Hypersensitivity in Mice
Abstract
:1. Introduction
2. Results
2.1. Decreased Neuronal Excitability and Sag Potential in vCA1 Pyramidal Neurons in Mice with CFA-Induced Inflammatory Pain
2.2. Decreased Expression of HCN2 in vCA1 of Mice with CFA-Induced Inflammatory Pain
2.3. Up/Down-Regulation of HCN2 in vCA1 Bidirectionally Modulates Nociceptive Hypersensitivity
2.4. Down-Regulation of HCN2 Decreased the Excitability of Pyramidal Neurons in vCA1 of Mice
2.5. Over-Expression of HCN2 in vCA1 Has Limited Effect on Anxiety-like Behaviors and Cognitive Impairment in Mice Injected with CFA
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Inflammatory Pain Model
4.3. Electrophysiological Recordings Ex Vivo
4.4. Immunostaining
4.5. Western Blot
4.6. HCN1/2 Regulation
4.7. Stereotaxic Injection
4.8. Thermal Pain Measurement
4.9. Object–Place Recognition (OPR)
4.10. Open Field Test (OFT)
4.11. The Elevated Plus Maze Test (EPM)
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zheng, Y.; Shao, S.; Zhang, Y.; Yuan, S.; Xing, Y.; Wang, J.; Qi, X.; Cui, K.; Tong, J.; Liu, F.; et al. HCN2 Channels in the Ventral Hippocampal CA1 Regulate Nociceptive Hypersensitivity in Mice. Int. J. Mol. Sci. 2023, 24, 13823. https://doi.org/10.3390/ijms241813823
Zheng Y, Shao S, Zhang Y, Yuan S, Xing Y, Wang J, Qi X, Cui K, Tong J, Liu F, et al. HCN2 Channels in the Ventral Hippocampal CA1 Regulate Nociceptive Hypersensitivity in Mice. International Journal of Molecular Sciences. 2023; 24(18):13823. https://doi.org/10.3390/ijms241813823
Chicago/Turabian StyleZheng, Yawen, Shan Shao, Yu Zhang, Shulu Yuan, Yuanwei Xing, Jiaxin Wang, Xuetao Qi, Kun Cui, Jifu Tong, Fengyu Liu, and et al. 2023. "HCN2 Channels in the Ventral Hippocampal CA1 Regulate Nociceptive Hypersensitivity in Mice" International Journal of Molecular Sciences 24, no. 18: 13823. https://doi.org/10.3390/ijms241813823