Nuciferine Inhibits Oral Squamous Cell Carcinoma Partially through Suppressing the STAT3 Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Nuciferine Inhibited the Proliferation and Promoted the Apoptosis of OSCC Cells
2.2. Nuciferine Inhibited the Migration and Invasion of OSCC Cells
2.3. STAT3 Signaling Pathway Was Suppressed in Nuciferine-Treated OSCC Cells
2.4. Nuciferine Inhibited the Proliferation and Promoted the Apoptosis of OSCC Cells via Suppressing the STAT3 Signaling Pathway
2.5. Nuciferine Inhibited the Migration and Invasion of OSCC Cells via Suppressing the STAT3 Signaling Pathway
2.6. Nuciferine Inhibited OSCC Growth via Suppressing the STAT3 Signaling Pathway
3. Discussion
4. Materials and Methods
4.1. Reagents Antibodies and Cell Culture
4.2. Cell Counting Kit-8 (CCK-8) Assay
4.3. Plate Colony-Forming Assay
4.4. Ethynyl-2-Deoxyuridine (EdU) Assay
4.5. Flow Cytometry
4.6. Wound-Healing Assay
4.7. Transwell Assay
4.8. Western Blot
4.9. Immunofluorescence
4.10. Cell-Derived Xenograft (CDX) Tumor Model
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Xie, J.-R.; Chen, X.-J.; Zhou, G. Nuciferine Inhibits Oral Squamous Cell Carcinoma Partially through Suppressing the STAT3 Signaling Pathway. Int. J. Mol. Sci. 2023, 24, 14532. https://doi.org/10.3390/ijms241914532
Xie J-R, Chen X-J, Zhou G. Nuciferine Inhibits Oral Squamous Cell Carcinoma Partially through Suppressing the STAT3 Signaling Pathway. International Journal of Molecular Sciences. 2023; 24(19):14532. https://doi.org/10.3390/ijms241914532
Chicago/Turabian StyleXie, Ji-Rong, Xiao-Jie Chen, and Gang Zhou. 2023. "Nuciferine Inhibits Oral Squamous Cell Carcinoma Partially through Suppressing the STAT3 Signaling Pathway" International Journal of Molecular Sciences 24, no. 19: 14532. https://doi.org/10.3390/ijms241914532