Transcriptional Signatures and Network-Based Approaches Identified Master Regulators Transcription Factors Involved in Experimental Periodontitis Pathogenesis
Abstract
:1. Introduction
2. Results
2.1. Experimental Model Confirmation: Alveolar Bone Loss
2.2. Global Expression Patterns and Differential Gene Expression (DGE) Analysis in Experimental Periodontitis
2.3. Gene Modules Associated with Periodontitis
2.4. Context-Specific Gene Regulatory Network on Experimental Periodontitis
2.5. Identification of Master Regulator Genes in Experimental Periodontitis
3. Discussion
4. Materials and Methods
4.1. Experimental Animals of the Study
4.2. Experimental Periodontitis Induction and Analysis of Alveolar Bone Loss
4.3. Indirect Immunofluorescence Confocal Assays
4.4. Total RNA Extraction
4.5. cDNA Synthesis and Gene Expression Analysis Using Quantitative Polymerase Chain Reaction (qRT-PCR)
4.6. RNA Library Preparation and Sequencing
4.7. RNA Sequencing [RNA-Seq] Data Analysis
4.8. Co-Expression Modules Analysis
4.9. Construction of Gene Regulatory Network
4.10. Identification of the Periodontitis-Master Regulators
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
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Vicencio, E.; Nuñez-Belmar, J.; Cardenas, J.P.; Cortés, B.I.; Martin, A.J.M.; Maracaja-Coutinho, V.; Rojas, A.; Cafferata, E.A.; González-Osuna, L.; Vernal, R.; et al. Transcriptional Signatures and Network-Based Approaches Identified Master Regulators Transcription Factors Involved in Experimental Periodontitis Pathogenesis. Int. J. Mol. Sci. 2023, 24, 14835. https://doi.org/10.3390/ijms241914835
Vicencio E, Nuñez-Belmar J, Cardenas JP, Cortés BI, Martin AJM, Maracaja-Coutinho V, Rojas A, Cafferata EA, González-Osuna L, Vernal R, et al. Transcriptional Signatures and Network-Based Approaches Identified Master Regulators Transcription Factors Involved in Experimental Periodontitis Pathogenesis. International Journal of Molecular Sciences. 2023; 24(19):14835. https://doi.org/10.3390/ijms241914835
Chicago/Turabian StyleVicencio, Emiliano, Josefa Nuñez-Belmar, Juan P. Cardenas, Bastian I. Cortés, Alberto J. M. Martin, Vinicius Maracaja-Coutinho, Adolfo Rojas, Emilio A. Cafferata, Luis González-Osuna, Rolando Vernal, and et al. 2023. "Transcriptional Signatures and Network-Based Approaches Identified Master Regulators Transcription Factors Involved in Experimental Periodontitis Pathogenesis" International Journal of Molecular Sciences 24, no. 19: 14835. https://doi.org/10.3390/ijms241914835