Evaluation of Oxidative Stress and Metabolic Profile in a Preclinical Kidney Transplantation Model According to Different Preservation Modalities
Abstract
:1. Introduction
2. Results
2.1. NMR and EPR Procedure
2.2. Analysis during the Preservation Procedure
1H-NMR Analysis from Preservation Solutions
2.3. Functional Analysis during the Survival Period
2.3.1. Early Functional Recovery
2.3.2. Oxidative Stress Evaluation
2.4. 1HNMR and EPR Tissue Analysis
2.5. Histological Evaluation
3. Discussion
4. Materials and Methods
4.1. Animal Model and Surgical Procedure
4.2. Post-Surgical Evaluation Procedure
4.3. Sampling Methods
4.3.1. Perfusate Sampling
4.3.2. Blood Sampling
4.3.3. Tissue Sampling
4.4. Measurements
4.4.1. EPR Measurements
4.4.2. Antioxidant Capacity
4.4.3. Enzymatic Assays
Thiobarbituric-Acid-Reactive Substances (TBARS)
Protein Carbonyls (PC)
4.4.4. 1H-NMR Analysis
4.5. Histological Evaluation
4.6. Renal Function Recovery Evaluation
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
ATP | Adenosine Triphosphate |
1H NMR | Proton Nuclear Magnetic Resonance |
CMH | 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine |
CP | 3-Carboxy-2,2,5,5-tetramethyl-1-pyrrolidinyloxy |
DCD | Donation after Cardiac Death |
DETC | Sodium Diethyldithio-Carbamate Trihydrate |
DF | Deferoxamine Methane-Sulfonate Salt |
DGF | Delayed Graft Function |
EPR | Electron Paramagnetic Resonance |
ECD | Expanded Criteria Donor |
HE | Hematoxylin and Eosin |
MDA | Malondialdehyde |
PC | Protein Carbonyls |
HMP | Hypothermic Machine Perfusion |
ROS | Reactive Oxygen Species |
SCD | Standard Criteria Donors |
SCS | Static Cold Storage |
TAC | Total Antioxidant Capacity |
TBARS | Thiobarbituric-Acid-Reactive Substances |
WIT | Warm Ischemia Time |
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Mrakic-Sposta, S.; Vezzoli, A.; Cova, E.; Ticcozzelli, E.; Montorsi, M.; Greco, F.; Sepe, V.; Benzoni, I.; Meloni, F.; Arbustini, E.; et al. Evaluation of Oxidative Stress and Metabolic Profile in a Preclinical Kidney Transplantation Model According to Different Preservation Modalities. Int. J. Mol. Sci. 2023, 24, 1029. https://doi.org/10.3390/ijms24021029
Mrakic-Sposta S, Vezzoli A, Cova E, Ticcozzelli E, Montorsi M, Greco F, Sepe V, Benzoni I, Meloni F, Arbustini E, et al. Evaluation of Oxidative Stress and Metabolic Profile in a Preclinical Kidney Transplantation Model According to Different Preservation Modalities. International Journal of Molecular Sciences. 2023; 24(2):1029. https://doi.org/10.3390/ijms24021029
Chicago/Turabian StyleMrakic-Sposta, Simona, Alessandra Vezzoli, Emanuela Cova, Elena Ticcozzelli, Michela Montorsi, Fulvia Greco, Vincenzo Sepe, Ilaria Benzoni, Federica Meloni, Eloisa Arbustini, and et al. 2023. "Evaluation of Oxidative Stress and Metabolic Profile in a Preclinical Kidney Transplantation Model According to Different Preservation Modalities" International Journal of Molecular Sciences 24, no. 2: 1029. https://doi.org/10.3390/ijms24021029