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Article

The Histone Chaperone Network Is Highly Conserved in Physarum polycephalum

by
Axel Poulet
1,†,
Ellyn Rousselot
2,†,
Stéphane Téletchéa
2,
Céline Noirot
3,
Yannick Jacob
1,
Josien van Wolfswinkel
1,
Christophe Thiriet
4 and
Céline Duc
2,*
1
Department of Molecular, Cellular and Developmental Biology, Faculty of Arts and Sciences, Yale University, New Haven, CT 06511, USA
2
Faculté des Sciences et Techniques, Nantes Université, CNRS, US2B, UMR 6286, 44000 Nantes, France
3
INRAE, UR 875 Unité de Mathématique et Informatique Appliquées, Genotoul Bioinfo Auzeville, 31326 Castanet-Tolosan, France
4
Université Rennes 1, CNRS, IGDR (Institut de Génétique et Développement de Rennes)—UMR 6290, 35043 Rennes, France
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2023, 24(2), 1051; https://doi.org/10.3390/ijms24021051
Submission received: 15 December 2022 / Revised: 30 December 2022 / Accepted: 2 January 2023 / Published: 5 January 2023
(This article belongs to the Special Issue Chromatin, Epigenetics and Plant Physiology 2.0)
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Abstract

The nucleosome is composed of histones and DNA. Prior to their deposition on chromatin, histones are shielded by specialized and diverse proteins known as histone chaperones. They escort histones during their entire cellular life and ensure their proper incorporation in chromatin. Physarum polycephalum is a Mycetozoan, a clade located at the crown of the eukaryotic tree. We previously found that histones, which are highly conserved between plants and animals, are also highly conserved in Physarum. However, histone chaperones differ significantly between animal and plant kingdoms, and this thus probed us to further study the conservation of histone chaperones in Physarum and their evolution relative to animal and plants. Most of the known histone chaperones and their functional domains are conserved as well as key residues required for histone and chaperone interactions. Physarum is divergent from yeast, plants and animals, but PpHIRA, PpCABIN1 and PpSPT6 are similar in structure to plant orthologues. PpFACT is closely related to the yeast complex, and the Physarum genome encodes the animal-specific APFL chaperone. Furthermore, we performed RNA sequencing to monitor chaperone expression during the cell cycle and uncovered two distinct patterns during S-phase. In summary, our study demonstrates the conserved role of histone chaperones in handling histones in an early-branching eukaryote.
Keywords: histone chaperones; protein domains; phylogeny; cell cycle; Physarum histone chaperones; protein domains; phylogeny; cell cycle; Physarum

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MDPI and ACS Style

Poulet, A.; Rousselot, E.; Téletchéa, S.; Noirot, C.; Jacob, Y.; van Wolfswinkel, J.; Thiriet, C.; Duc, C. The Histone Chaperone Network Is Highly Conserved in Physarum polycephalum. Int. J. Mol. Sci. 2023, 24, 1051. https://doi.org/10.3390/ijms24021051

AMA Style

Poulet A, Rousselot E, Téletchéa S, Noirot C, Jacob Y, van Wolfswinkel J, Thiriet C, Duc C. The Histone Chaperone Network Is Highly Conserved in Physarum polycephalum. International Journal of Molecular Sciences. 2023; 24(2):1051. https://doi.org/10.3390/ijms24021051

Chicago/Turabian Style

Poulet, Axel, Ellyn Rousselot, Stéphane Téletchéa, Céline Noirot, Yannick Jacob, Josien van Wolfswinkel, Christophe Thiriet, and Céline Duc. 2023. "The Histone Chaperone Network Is Highly Conserved in Physarum polycephalum" International Journal of Molecular Sciences 24, no. 2: 1051. https://doi.org/10.3390/ijms24021051

APA Style

Poulet, A., Rousselot, E., Téletchéa, S., Noirot, C., Jacob, Y., van Wolfswinkel, J., Thiriet, C., & Duc, C. (2023). The Histone Chaperone Network Is Highly Conserved in Physarum polycephalum. International Journal of Molecular Sciences, 24(2), 1051. https://doi.org/10.3390/ijms24021051

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