Chondrocyte Isolation from Loose Bodies—An Option for Reducing Donor Site Morbidity for Autologous Chondrocyte Implantation

Textor, Martin; Hoburg, Arnd; Lehnigk, Rex; Perka, Carsten; Duda, Georg N.; Reinke, Simon; Blankenstein, Antje; Hochmann, Sarah; Stockinger, Andreas; Resch, Herbert; Wolf, Martin; Strunk, Dirk; Geissler, Sven

doi:10.3390/ijms24021484

Open AccessArticle

Chondrocyte Isolation from Loose Bodies—An Option for Reducing Donor Site Morbidity for Autologous Chondrocyte Implantation

by

Martin Textor

^1,2,

Arnd Hoburg

^2,3,4,

Rex Lehnigk

^2,3,

Carsten Perka

^1,2,3

,

Georg N. Duda

^1,2,3,5,

Simon Reinke

^1,2

,

Antje Blankenstein

^1,2,

Sarah Hochmann

⁶

,

Andreas Stockinger

⁷,

Herbert Resch

⁸,

Martin Wolf

⁶,

Dirk Strunk

⁶

and

Sven Geissler

^1,2,9,*

¹

Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité—Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany

²

Julius Wolff Institute, Berlin Institute of Health at Charité—Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany

³

Centrum für Muskuloskelettale Chirugie (CBMSC), Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Augustenburger Platz 1, 13353 Berlin, Germany

⁴

Med Center 360 Degree Berlin, Kieler Straße 1, 12163 Berlin, Germany

⁵

Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02138, USA

⁶

Cell Therapy Institute, Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University (PMU), 5020 Salzburg, Austria

⁷

Department of Traumatology, Clinic Schaerding, 4780 Schaerding, Austria

⁸

Department of Traumatology, Paracelsus Medical University (PMU), 5020 Salzburg, Austria

⁹

Berlin Center for Advanced Therapies (BECAT), Charité Universitätsmedizin Berlin, 13353 Berlin, Germany

^*

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2023, 24(2), 1484; https://doi.org/10.3390/ijms24021484

Submission received: 30 November 2022 / Revised: 21 December 2022 / Accepted: 31 December 2022 / Published: 12 January 2023

(This article belongs to the Special Issue Molecular Metabolisms in Cartilage Health and Diseases 2.0)

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Abstract

Loose bodies (LBs) from patients with osteochondritis dissecans (OCD) are usually removed and discarded during surgical treatment of the defect. In this study, we address the question of whether these LBs contain sufficient viable and functional chondrocytes that could serve as a source for autologous chondrocyte implantation (ACI) and how the required prolonged in vitro expansion affects their phenotype. Chondrocytes were isolated from LBs of 18 patients and compared with control chondrocyte from non-weight-bearing joint regions (n = 7) and bone marrow mesenchymal stromal cells (BMSCs, n = 6) obtained during primary arthroplasty. No significant differences in the initial cell yield per isolation and the expression of the chondrocyte progenitor cell markers CD44 + /CD146+ were found between chondrocyte populations from LBs (LB-CH) and control patients (Ctrl-CH). During long-term expansion, LB-CH exhibited comparable viability and proliferation rates to control cells and no ultimate cell cycle arrest was observed within 12 passages respectively 15.3 ± 1.1 mean cumulative populations doublings (CPD). The chondrogenic differentiation potential was comparable between LB-CH and Ctrl-CH, but both groups showed a significantly higher ability to form a hyaline cartilage matrix in vitro than BMSC. Our data suggest that LBs are a promising cell source for obtaining qualitatively and quantitatively suitable chondrocytes for therapeutic applications, thereby circumventing donor site morbidity as a consequence of the biopsies required for the current ACI procedure.

Keywords: cartilage; human chondrocytes; loose body; regeneration; ACI; OCD; BMSC

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MDPI and ACS Style

Textor, M.; Hoburg, A.; Lehnigk, R.; Perka, C.; Duda, G.N.; Reinke, S.; Blankenstein, A.; Hochmann, S.; Stockinger, A.; Resch, H.; et al. Chondrocyte Isolation from Loose Bodies—An Option for Reducing Donor Site Morbidity for Autologous Chondrocyte Implantation. Int. J. Mol. Sci. 2023, 24, 1484. https://doi.org/10.3390/ijms24021484

AMA Style

Textor M, Hoburg A, Lehnigk R, Perka C, Duda GN, Reinke S, Blankenstein A, Hochmann S, Stockinger A, Resch H, et al. Chondrocyte Isolation from Loose Bodies—An Option for Reducing Donor Site Morbidity for Autologous Chondrocyte Implantation. International Journal of Molecular Sciences. 2023; 24(2):1484. https://doi.org/10.3390/ijms24021484

Chicago/Turabian Style

Textor, Martin, Arnd Hoburg, Rex Lehnigk, Carsten Perka, Georg N. Duda, Simon Reinke, Antje Blankenstein, Sarah Hochmann, Andreas Stockinger, Herbert Resch, and et al. 2023. "Chondrocyte Isolation from Loose Bodies—An Option for Reducing Donor Site Morbidity for Autologous Chondrocyte Implantation" International Journal of Molecular Sciences 24, no. 2: 1484. https://doi.org/10.3390/ijms24021484

APA Style

Textor, M., Hoburg, A., Lehnigk, R., Perka, C., Duda, G. N., Reinke, S., Blankenstein, A., Hochmann, S., Stockinger, A., Resch, H., Wolf, M., Strunk, D., & Geissler, S. (2023). Chondrocyte Isolation from Loose Bodies—An Option for Reducing Donor Site Morbidity for Autologous Chondrocyte Implantation. International Journal of Molecular Sciences, 24(2), 1484. https://doi.org/10.3390/ijms24021484

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Chondrocyte Isolation from Loose Bodies—An Option for Reducing Donor Site Morbidity for Autologous Chondrocyte Implantation

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