Characterization of Transcriptome Dynamics during Early Fruit Development in Olive (Olea europaea L.)
Abstract
:1. Introduction
2. Results and Discussion
2.1. Morphological and Cytological Changes during Early Fruit Development in Olive
2.2. Ploidy Level during Early Fruit Development in Olive
2.3. Overall Transcriptional Changes during Early Fruit Development in Olive
2.4. Gene Ontology Functional Enrichment Analysis of Differentially Expressed Genes
2.5. Characterization of Cell Cycle-Related Genes Associated with Early Fruit Development in Olive
2.6. Differing Hormonal Composition and Gene Expression Patterns during Early Fruit Development in Olive
2.7. Signaling Peptides Regulating Early Fruit Development in Olive
2.8. Transcript Changes in Cell Wall Biosynthesis and Remodeling during Early Olive Fruit Development
2.9. Characterization of Transport-Related Genes Associated with Early Olive Fruit Development
2.10. Identifying Transcription Factors Critical for Early Olive Fruit Development
3. Materials and Methods
3.1. Plant Material and Cytological Study
3.2. Flow Cytometry Analysis
3.3. RNA Extraction, Library Preparation, and Sequencing
3.4. Differential Expression
3.5. Quantitative RT-PCR
3.6. Quantification of Plant Hormones
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
ABA | Abscisic acid |
ACC | 1-Aminocyclopropane-1-carboxylic acid |
ABC | ATP-binding cassette transporter |
AGP | Arabinogalactan protein |
ARFs | Auxin response factors |
BG | Glucan 1,3-β-glucosidase |
β-GAL | β-galactosidase |
BR | Brassinosteroid |
BRI1 | BR insensitive 1 |
BAK1 | BRI1-associated kinase1 |
BIN2 | BR insensitive 2 |
BRZ1 | Brassinazole resistant 1 |
CALS | Callose synthase |
cDNA | Complementary deoxyribonucleic acid |
CDK | Cyclin-dependent kinase |
CES | Cellulose synthase |
CHI | Chitinase |
CK | Cytokinin |
CKI | CDK inhibitor protein |
CKX | CK oxidase/dehydrogenase |
CYC | cyclin |
DEG | Differentially expressed gene |
DPA | Days post-anthesis |
EGase/CEL | Endo-1,4-β-glucanase or cellulase |
ERF | Ethylene response factor |
ET | Ethylene |
EXP | Expansin |
EXT | Extensin |
GA | Gibberellin |
GID1 | Gibberellin-insensitive dwarf1 |
GO | Gene Ontology |
IAA | Indole-3-acetic acid |
JA | Jasmonic acid |
KT | Potassium transporter |
LAC | Laccase |
LOG | Cytokinin riboside 5’-monophosphate phosphoribohydrolase |
PA | Polyamine |
PG | Polygalacturonase |
PME | Pectin methylesterase |
qRT-PCR | Quantitative real-time polymerase chain reaction |
RB | Retinoblastoma protein |
SA | Salicylic acid |
SAUR | Small auxin up RNA |
SL | Strigolactone |
SBT | Subtilase |
TF | Transcription factor |
TIR1 | Transport inhibitor response 1 |
tZ | trans-Zeatin |
XTH | Xyloglucan endotransglucosylase/hydrolase |
ZNT | Zinc transporter |
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Camarero, M.C.; Briegas, B.; Corbacho, J.; Labrador, J.; Gallardo, M.; Gomez-Jimenez, M.C. Characterization of Transcriptome Dynamics during Early Fruit Development in Olive (Olea europaea L.). Int. J. Mol. Sci. 2023, 24, 961. https://doi.org/10.3390/ijms24020961
Camarero MC, Briegas B, Corbacho J, Labrador J, Gallardo M, Gomez-Jimenez MC. Characterization of Transcriptome Dynamics during Early Fruit Development in Olive (Olea europaea L.). International Journal of Molecular Sciences. 2023; 24(2):961. https://doi.org/10.3390/ijms24020961
Chicago/Turabian StyleCamarero, Maria C., Beatriz Briegas, Jorge Corbacho, Juana Labrador, Mercedes Gallardo, and Maria C. Gomez-Jimenez. 2023. "Characterization of Transcriptome Dynamics during Early Fruit Development in Olive (Olea europaea L.)" International Journal of Molecular Sciences 24, no. 2: 961. https://doi.org/10.3390/ijms24020961