The Responses of Sucrose Metabolism and Carbon Translocation in Tomato Seedlings under Different Light Spectra
Abstract
:1. Introduction
2. Results
2.1. Effects of Light Spectra on the Growth of Tomato Seedlings
2.2. The Responses of Photosynthetic Performance of Tomato Seedlings under Different Light Spectra
2.3. Carbohydrate Contents in Plants under Different Light Spectra
2.4. Organic Carbon and Isotope Composition of Carbon in Plants under Different Light Spectra
2.5. The Distribution of Photosynthetic Products under Different Light Spectra
2.6. Sucrose Metabolism Enzyme Activities and Related Gene Expression
3. Discussion
4. Materials and Methods
4.1. Materials and Growth Conditions
4.2. Light Treatment
4.3. Gas Exchange Measurement and Sample Collection
4.4. Biomass and Plant Growth Determination
4.5. Carbohydrate Metabolism Enzyme Activity Measurement
4.6. Carbohydrate Content Measurement
4.7. Pulse-Labeling Experiments and Analysis of Stable Carbon Isotope Composition
4.8. Total RNA Extraction and qPCR Analysis
4.9. Data Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
B | R | RB | W | |
---|---|---|---|---|
UV-A light (380–400 nm) | 0 | 0 | 0 | 5.47 ± 0.32 |
Blue light (401–500 nm) | 300 ± 8.79 | 0 | 150 ± 4.16 | 65.42 ± 3.34 |
Green light (501–600 nm) | 0 | 0 | 0 | 110.82 ± 4.17 |
Red light (601–700 nm) | 0 | 300 ± 10.42 | 150 ± 6.31 | 127.76 ± 6.45 |
Far-red light (701–780 nm) | 0 | 0 | 0 | 5.29 ± 0.27 |
PPFD (400–700 nm) | 300 ± 8.79 | 300 ± 10.42 | 300 ± 11.23 | 300 ± 13.10 |
Total light intensity | 300 ± 8.79 | 300 ± 10.42 | 300 ± 11.23 | 314.76 ± 14.54 |
Gene Name | Gene ID: | Sequences |
---|---|---|
SPS | AB051216 | Forward: GCTTGGACGGGATTCTGATA |
Reverse: TCTCCCCCATCTCACTCATC | ||
SS3 | LOC543961 | Forward: GGATTGAAAGCCACGGAAAAGG |
Reverse: ACCAGGCCTCAAACGAATAGCA | ||
VI | AF465612 | Forward: TCCGCCTCTCGTTACACATTACTC |
Reverse: CGGTGACTGGTTGTTGAGGATAGG | ||
NI6 | 100134879 | Forward: GGAAGCTGCTCCATCCTTGT |
Reverse: GACGTGGCGGTGAATGAGT |
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Leaf Area (cm−2) | Dry Weight (DW, g) | Biomass Distribution Ratio | ||||||
---|---|---|---|---|---|---|---|---|
Leaves | Stems | Roots | ||||||
W | 325.62 ± 46.89 b | 1.11 ± 0.10 b | 53.31 ± 0.80% a | 37.09 ± 0.64% d | 9.60 ± 0.51% b | |||
RB | 476.09 ± 53.4 1 a | 1.79 ± 0.26 a | 43.78 ± 2.1% c | −9.53% | 44.01 ± 1.1% a | 6.92% | 12.21 ± 0.53% a | 2.61% |
R | 266.01 ± 38.25 bc | 0.93 ± 0.04 c | 47.74 ± 2.6% b | −7.57% | 40.80 ± 0.40% c | 3.71% | 11.46 ± 1.2% ab | 1.86% |
B | 205.86 ± 20.17 c | 0.87 ± 0.08 c | 48.30 ± 1.1% b | −5.01% | 41.96 ± 0.61% b | 4.87% | 9.74 ± 1.3% b | 0.1% |
W | RB | R | B | |
---|---|---|---|---|
Leaves (mg g−1) | ||||
Glucose | 7.33 ± 0.22 d | 18.69 ± 0.35 a | 10.62 ± 0.30 b | 8.35 ± 0.41 c |
Sucrose | 6.40 ± 0.10 c | 8.34 ± 0.31 a | 7.64 ± 0.32 b | 4.88 ± 0.26 d |
Starch | 5.08 ± 0.16 b | 8.40 ± 0.09 a | 5.34 ± 0.20 b | 4.56 ± 0.23 c |
Stems (mg g−1) | ||||
Glucose | 11.34 ± 0.24 b | 20.86 ± 0.55 a | 11.19 ± 0.28 b | 8.05 ± 0.31 c |
Sucrose | 1.70 ± 0.20 c | 3.31 ± 0.07 a | 2.03 ± 0.01 b | 1.71 ± 0.09 c |
Starch | 4.12 ± 0.04 b | 6.88 ± 0.28 a | 3.33 ± 0.05 d | 3.69 ± 0.02 c |
Roots (mg g−1) | ||||
Glucose | 8.69 ± 0.23 b | 9.50 ± 0.36 a | 8.61 ± 0.35 b | 8.91 ± 0.03 b |
Sucrose | 5.26 ± 0.11 a | 3.44 ± 0.11 b | 3.20 ± 0.07 b | 2.18 ± 0.09 d |
Starch | 4.33 ± 0.09 a | 3.13 ± 0.94 bc | 3.55 ± 0.05 b | 3.03 ± 0.06 c |
Treatments | Organic Carbon (g g−1) | δ13C (‰) | ||||
---|---|---|---|---|---|---|
Leaves | Stems | Roots | Leaves | Stems | Roots | |
W | 0.72 ± 0.04 a | 0.59 ± 0.05 c | 0.62 ± 0.02 b | −41.61 ± 0.78 a | −42.43 ± 1.21 d | −31.99 ± 0.78 b |
RB | 0.69 ± 0.07 a | 0.66 ± 0.02 a | 0.80 ± 0.04 a | −41.64 ±1.53 a | −31.14 ± 0.91 b | −36.50 ± 0.45 c |
R | 0.79 ± 0.08 a | 0.64 ± 0.07 b | 0.69 ± 0.05 b | −41.27 ± 0.54 a | −27.86 ±1.08 a | −16.93 ± 0.19 a |
B | 0.67 ± 0.06 a | 0.61 ± 0.05 b | 0.71 ± 0.07 b | −49.53 ± 0.24 b | −35.50 ± 2.13 c | −45.53 ± 0.31 d |
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Zhan, X.; Yang, Q.; Wang, S.; Wang, Y.; Fan, X.; Bian, Z. The Responses of Sucrose Metabolism and Carbon Translocation in Tomato Seedlings under Different Light Spectra. Int. J. Mol. Sci. 2023, 24, 15054. https://doi.org/10.3390/ijms242015054
Zhan X, Yang Q, Wang S, Wang Y, Fan X, Bian Z. The Responses of Sucrose Metabolism and Carbon Translocation in Tomato Seedlings under Different Light Spectra. International Journal of Molecular Sciences. 2023; 24(20):15054. https://doi.org/10.3390/ijms242015054
Chicago/Turabian StyleZhan, Xiaoxu, Qichang Yang, Sen Wang, Yu Wang, Xiaoxue Fan, and Zhonghua Bian. 2023. "The Responses of Sucrose Metabolism and Carbon Translocation in Tomato Seedlings under Different Light Spectra" International Journal of Molecular Sciences 24, no. 20: 15054. https://doi.org/10.3390/ijms242015054