Studying the Association of TKS4 and CD2AP Scaffold Proteins and Their Implications in the Partial Epithelial–Mesenchymal Transition (EMT) Process
Abstract
:1. Introduction
2. Results
2.1. CD2AP and TKS4 Form a Scaffolding Protein Complex
2.2. Absence of CD2AP and TKS4 Promotes Colon Cancer Cell Migration Separately and Simultaneously via a Partial Epithelial–Mesenchymal Transition Process
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. siRNA and DNA Plasmid Transfection
4.3. RNA Isolation and Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
4.4. Western Blotting and Immunoprecipitation
4.5. Mass Spectrometry Analyses
4.6. In-Gel Protein Digestion
4.7. Liquid Chromatography-Mass Spectrometry Analysis
4.8. Protein Identification
4.9. Cell Proliferation Assay
4.10. Wound Healing Assays
4.11. Immunocytochemistry
4.12. DuoLink Proximity Ligation Assays
4.13. Peptide Array
4.14. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kurilla, A.; László, L.; Takács, T.; Tilajka, Á.; Lukács, L.; Novák, J.; Pancsa, R.; Buday, L.; Vas, V. Studying the Association of TKS4 and CD2AP Scaffold Proteins and Their Implications in the Partial Epithelial–Mesenchymal Transition (EMT) Process. Int. J. Mol. Sci. 2023, 24, 15136. https://doi.org/10.3390/ijms242015136
Kurilla A, László L, Takács T, Tilajka Á, Lukács L, Novák J, Pancsa R, Buday L, Vas V. Studying the Association of TKS4 and CD2AP Scaffold Proteins and Their Implications in the Partial Epithelial–Mesenchymal Transition (EMT) Process. International Journal of Molecular Sciences. 2023; 24(20):15136. https://doi.org/10.3390/ijms242015136
Chicago/Turabian StyleKurilla, Anita, Loretta László, Tamás Takács, Álmos Tilajka, Laura Lukács, Julianna Novák, Rita Pancsa, László Buday, and Virág Vas. 2023. "Studying the Association of TKS4 and CD2AP Scaffold Proteins and Their Implications in the Partial Epithelial–Mesenchymal Transition (EMT) Process" International Journal of Molecular Sciences 24, no. 20: 15136. https://doi.org/10.3390/ijms242015136