Regulation Mechanism of Dopamine Receptor 1 in Low Temperature Response of Marsupenaeus japonicus
Abstract
:1. Introduction
2. Results
2.1. Characterization of the MjDAD1 Sequence
2.2. Tissue Expression of MjDAD1
2.3. MjDAD1 mRNA Expression under Low Temperature Stress
2.4. Low Temperature Stress Effects on Intracellular Pathway Factors
2.4.1. Effects of Low Temperature Stress on G Protein Effectors
2.4.2. Effect of Low Temperature Stress on Second Messengers
2.4.3. Effect of Low Temperature Stress on Protein Kinases
2.5. The Relative Expression Ratio of MjDAD1 after MjDAD1 RNAi
2.6. Effect of MjDAD1 Interference on Tissue Damage of M. japonicus under Low Temperature Stress
2.6.1. Histopathology of the Gills and Hepatopancreas after MjDAD1 Interference
2.6.2. TUNEL Detection of Apoptosis after MjDAD1 Interference
2.7. MjDAD1 Silencing Affects Intracellular Signaling Pathway Factors in the Gills and Hepatopancreas under Low Temperature Stress
2.8. MjDAD1 Silencing Affects Apoptosis-related Gene Expression in M. japonicus under Low Temperature Stress
3. Discussion
4. Materials and Methods
4.1. Ethical Considerations
4.2. Experimental Animals
4.3. Application of Low Temperature Stress and Collection of Samples
4.4. Cloning of MjDAD1
4.4.1. Extraction of Total RNA and Full Length cDNA Cloning
4.4.2. Analysis of the Sequence and Phylogenetic Tree Construction
4.4.3. The Distribution of MjDAD1 in Tissues and Analysis of Its Expression Pattern
4.5. Short Interfering RNA (siRNA) Analysis
4.6. Histological Examination and TUNEL Assay
4.7. Protein Kinases and Intracellular Second Messengers Determination
4.7.1. Supernatant Preparations
4.7.2. Assay for Intracellular Signaling Transduction Factors
4.8. Quantitative Real-Time Reverse Transcription PCR (qRT-PCR)
4.9. Statistical Considerations
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
GPCRs | G protein-coupled receptors |
DARs | Dopamine receptors |
DAD1 | D1-Dopamine receptor |
DA | dopamine |
AC | adenylate cyclase |
PLC | phospholipase C |
cAMP | cyclic adenosine monophosphate |
cGMP | cyclic guanosine monophosphate |
CaM | calmodulin |
DAG | diacylglycerol |
PKA | protein kinase A |
PKC | protein kinases C |
Bcl-2 | BCL2 apoptosis regulator |
TM | transmembrane |
TUNEL | Terminal deoxynulceotidyl transferase nick-end-labeling |
qRT-PCR | quantitative real-time reverse transcription PCR |
PBS | Phosphate-buffered saline |
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Primer | Sequence | Usage |
---|---|---|
MjDAD1 F1 | ATTCCCGACATCGTTTTCAAGGTGC | 3′ RACE |
MjDAD1 F2 | CAGTTCTTCTTCTGCTTACGCCACCC | 3′ RACE |
MjDAD1 R1 | GGCAAAGGTCATCACCACGCA | 5′ RACE |
MjDAD1 R2 | AGGAAGATGACGAAGGAGAGGATGATGC | 5′ RACE |
UPM(short) | CTAATACGACTCACTATAGGGC | RACE |
UPM(long) | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | RACE |
M13 F | GTAAAACGACGGCCAGT | colony PCR |
M13 R | CAGGAAACAGCTATGAC | colony PCR |
NUP | AAGCAGTGGTATCAACGCAGAGT | RACE |
dsDAD1 F | GCUUGAUAUCAAUCUUCUATT | RNAi |
dsDAD1 R | UAGAAGAGUGAUAUCAAGCTT | RNAi |
NC F | UUCUCCGAACGUGUCACGUTT | RNAi |
NC R | ACGUGACACGUUCGGAGAATT | RNAi |
MjDAD1 F | CGCCTCCATCATCAACCTCT | qRT-PCR |
MjDAD1 R | GCCATCGTCACGATCCTCTT | qRT-PCR |
MjDAD2 R | AAGCAAGCACGTCGAAACTCC | qRT-PCR |
MjBcl-2 F | TCTCAAAATGGCTCCCG | qRT-PCR |
MjBcl-2 R | GTCACTGTCGCTCACACTAC | qRT-PCR |
p53 F | CCAGTGGGTGGAGTATCA | qRT-PCR |
p53 R | TTTGTGACGACCAGCCC | qRT-PCR |
caspase-3 F | GCCTCTCACGACGCCTACAT | qRT-PCR |
caspase-3 R | GTCGCTGTGGTCTCGTT | qRT-PCR |
β-actin F | TCCACGAGACCACATACAAC | qRT-PCR |
β-actin R | CACTTCCTGTGAACGATTGA | qRT-PCR |
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Ren, X.; Bian, X.; Shao, H.; Jia, S.; Yu, Z.; Liu, P.; Li, J.; Li, J. Regulation Mechanism of Dopamine Receptor 1 in Low Temperature Response of Marsupenaeus japonicus. Int. J. Mol. Sci. 2023, 24, 15278. https://doi.org/10.3390/ijms242015278
Ren X, Bian X, Shao H, Jia S, Yu Z, Liu P, Li J, Li J. Regulation Mechanism of Dopamine Receptor 1 in Low Temperature Response of Marsupenaeus japonicus. International Journal of Molecular Sciences. 2023; 24(20):15278. https://doi.org/10.3390/ijms242015278
Chicago/Turabian StyleRen, Xianyun, Xueqiong Bian, Huixin Shao, Shaoting Jia, Zhenxing Yu, Ping Liu, Jian Li, and Jitao Li. 2023. "Regulation Mechanism of Dopamine Receptor 1 in Low Temperature Response of Marsupenaeus japonicus" International Journal of Molecular Sciences 24, no. 20: 15278. https://doi.org/10.3390/ijms242015278