ERp29 Attenuates Nicotine-Induced Endoplasmic Reticulum Stress and Inhibits Choroidal Neovascularization
Abstract
:1. Introduction
2. Results
2.1. Nicotine Exacerbates ER Stress and Upregulates the Expression of ERp29 in ARPE-19 Cells
2.2. ERp29 Attenuates Nicotine-Induced ER Stress and Protects ARPE-19 Cells
2.3. Knockdown of ERp29 Expression Exacerbates Nicotine-Induced ER Stress and Decreases the Viability of ARPE-19 Cells
2.4. ERp29 Regulates Macrophage Polarization In Vitro
2.5. ERp29 Inhibited HUVECs Proliferation, Migration, and Tube Formation In Vitro
2.6. ERp29 Inhibits the Activity and Growth of Mouse CNV In Vivo
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Cell Treatment
4.2. Construction and Transduction of Lentivirus
4.3. Small-Interfering RNAs (siRNAs)
4.4. Cell Viability and Proliferation Assays
4.5. Western Blotting Analysis
4.6. RNA Isolation and Quantitative PCR (qPCR)
4.7. Macrophage Polarization
4.8. ARPE-19 Cell/M2 Macrophage Coculture System
4.9. Wound-Healing Assay
4.10. Tube Formation Assay
4.11. Mouse Model of CNV and Administration
4.12. Fundus Fluorescein Angiography (FFA)
4.13. Choroidal Flat Mount and Immunostaining
4.14. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lu, T.; Xie, F.; Huang, C.; Zhou, L.; Lai, K.; Gong, Y.; Li, Z.; Li, L.; Liang, J.; Cong, Q.; et al. ERp29 Attenuates Nicotine-Induced Endoplasmic Reticulum Stress and Inhibits Choroidal Neovascularization. Int. J. Mol. Sci. 2023, 24, 15523. https://doi.org/10.3390/ijms242115523
Lu T, Xie F, Huang C, Zhou L, Lai K, Gong Y, Li Z, Li L, Liang J, Cong Q, et al. ERp29 Attenuates Nicotine-Induced Endoplasmic Reticulum Stress and Inhibits Choroidal Neovascularization. International Journal of Molecular Sciences. 2023; 24(21):15523. https://doi.org/10.3390/ijms242115523
Chicago/Turabian StyleLu, Tu, Fangfang Xie, Chuangxin Huang, Lijun Zhou, Kunbei Lai, Yajun Gong, Zijing Li, Longhui Li, Jiandong Liang, Qifeng Cong, and et al. 2023. "ERp29 Attenuates Nicotine-Induced Endoplasmic Reticulum Stress and Inhibits Choroidal Neovascularization" International Journal of Molecular Sciences 24, no. 21: 15523. https://doi.org/10.3390/ijms242115523