Some Structural Elements of Bacterial Protein MF3 That Influence Its Ability to Induce Plant Resistance to Fungi, Viruses, and Other Plant Pathogens
Abstract
:1. Introduction
2. Results
2.1. Design of AC MF3 Mutants
2.2. Inducing Activity of AC MF3 Mutants
2.3. The 3D Model of MF3 and Its Analysis
3. Discussion
4. Materials and Methods
4.1. Design and Obtaining of AC MF3 and Alpha-Helix Deletion Mutants
4.2. Inducing Activity Assay
4.3. The 2D Structure Prediction and 3D Modeling
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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No. | Primer | 5′–3′ Sequence |
---|---|---|
1 | 2 | 3 |
1 | 11(G_A)F | cctggaagccaaagctgtcggcg |
2 | 11(G_A)R | ctttggcttccagggctttttc |
3 | 17(D_A)F | tcggcgacgccttggaagtggc |
4 | 17(D_A)R | acttccaaggcgtcgccgacag |
5 | 18(L_A)F | gcgacgacgcggaagtggccgttg |
6 | 18(L_A)R | ccacttccgcgtcgtcgccgacag |
7 | 20(V_A)F | ttggaagcggccgttgagcc |
8 | 20(V_A)R | aacggccgcttccaagtcgtc |
9 | 22(V_A)F | gtggccgctgagccggaagatg |
10 | 22(V_A)R | tccggctcagcggccacttccaag |
11 | 24(P_A)F | gttgaggcggaagatgcttacg |
12 | 24(P_A)R | tcttccgcctcaacggccacttc |
13 | 25(E_A)F | ttgagccggcagatgcttacggc |
14 | 25(E_A)R | aagcatctgccggctcaacggcc |
15 | 26(D_A)F | cggaagctgcttacggcgaatac |
16 | 26(D_A)R | gtaagcagcttccggctcaacgg |
17 | 28(Y_L)R | gatgctttaggcgaatacgccg |
18 | 28(Y_L)F | attcgcctaaagcatcttccgg |
19 | 29(G_A)F | tgcttacgccgaatacgccgcc |
20 | 29(G_A)R | cgtattcggcgtaagcatcttcc |
21 | MF3_dAs_F | AggTgCAggCAACATCATCggCAAAgCTgTCggCgACgAgcTgga |
22 | MF3_dAs_R | gATgATgTTgCCTgCACCTTgCAggTAAACCAgCggAgCgC |
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Erokhin, D.; Popletaeva, S.; Sinelnikov, I.; Rozhkova, A.; Shcherbakova, L.; Dzhavakhiya, V. Some Structural Elements of Bacterial Protein MF3 That Influence Its Ability to Induce Plant Resistance to Fungi, Viruses, and Other Plant Pathogens. Int. J. Mol. Sci. 2023, 24, 16374. https://doi.org/10.3390/ijms242216374
Erokhin D, Popletaeva S, Sinelnikov I, Rozhkova A, Shcherbakova L, Dzhavakhiya V. Some Structural Elements of Bacterial Protein MF3 That Influence Its Ability to Induce Plant Resistance to Fungi, Viruses, and Other Plant Pathogens. International Journal of Molecular Sciences. 2023; 24(22):16374. https://doi.org/10.3390/ijms242216374
Chicago/Turabian StyleErokhin, Denis, Sophya Popletaeva, Igor Sinelnikov, Alexandra Rozhkova, Larisa Shcherbakova, and Vitaly Dzhavakhiya. 2023. "Some Structural Elements of Bacterial Protein MF3 That Influence Its Ability to Induce Plant Resistance to Fungi, Viruses, and Other Plant Pathogens" International Journal of Molecular Sciences 24, no. 22: 16374. https://doi.org/10.3390/ijms242216374