Stem Cell Responsiveness to Imatinib in Chronic Myeloid Leukemia
Abstract
:1. Introduction
2. Results
2.1. BCR-ABL Expression in Leukemic LHSCs and LVSELs
2.2. Quantification of Lin−CD34+ Stem Cell Populations’ Responsiveness to IM
2.3. Quantification of Stem Cell Survival
2.4. Quantification of Gene Expression
2.5. miRNA Expression in Lin−CD34+CD45+ and Lin−CD34+CD45− CML Stem Cells
3. Discussion
4. Materials and Methods
4.1. Stem Cell Expansion
4.2. Flow Cytometry Analysis
4.3. RNA Isolation and Quantitative RT-PCR
4.4. MicroRNA Quantification
4.5. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Reference Range (M/F) # | Diagnosis (n = 16) | Follow Up (n = 20) | |
---|---|---|---|
Gender (M/F) | - | (10/6) | (14/6) |
Age (year) | - | 57 ± 16 | 56 ± 15 |
WBC (×109/L) | 3.9–10.9/4.9–12.68 | 92 ± 76 | 6 ± 2 |
RBC (×1012/L) | 4.44–5.61/3.92–5.08 | 4 ± 1 | 4 ± 1 |
Hgb (g/dL) | 13.5–16.9/11.9–14.6 | 12 ± 2 | 13 ± 3 |
hematocrit | 40.0–49.4/36.6–44.0 | 38 ± 5 | 38 ± 10 |
PLT (×109/L) | 166–308/173–390 | 586 ± 560 | 204 ± 91 |
Primer Sequences | |
---|---|
BCR ABL M Fw | TCC GCT GAC CAT CAA TAA GGA |
ABL Rv | CAC TCA GAC CCT GAG GCT CAA |
Probe BCRABL | [6-FAM] CCC TTC AGC GGC CAG TAG CAT CTG A [BHQ1] |
ABL1 Fw | TGG AGA TAA CAC TCT AAG CAT AAC TAA AGG |
ABL2 Rv | GAT GTA GTT GCT TGG GAC CCA |
Probe ABL | [6-FAM] CCA TTT TTG GTT TGG GCT TCA CAC CAT T [BHQ1] |
KI67 Fw | ATTGAACCTGCGGAAGAGCTGA |
KI67 Rv | GGAGCGCAGGGATATTCCCTTA |
P57 Kip2 Fw | GCGGCGATCAAGAAGCTGT |
P57 Kip2 Rv | TGGCGAAGAAATCGGAGATCA |
p21 Cip1 Fw | TGTCCGTCAGAACCCATGC |
p21 Cip1 Rv | AAAGTCGAAGTTCCATCGCTC |
GAPDH Fw | CATCGCTCAGACACCATGG |
GAPDH Rv | ATGTAGTTGAGGTCAATGAAGGG |
β2-microglobulin Fw | TGACTTTGTCACAGCCCAAGATA |
β2-microglobulin Rv | AATGCGGCATCTTCAAACCT |
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Lahlil, R.; Aries, A.; Scrofani, M.; Zanetti, C.; Hennequin, D.; Drénou, B. Stem Cell Responsiveness to Imatinib in Chronic Myeloid Leukemia. Int. J. Mol. Sci. 2023, 24, 16671. https://doi.org/10.3390/ijms242316671
Lahlil R, Aries A, Scrofani M, Zanetti C, Hennequin D, Drénou B. Stem Cell Responsiveness to Imatinib in Chronic Myeloid Leukemia. International Journal of Molecular Sciences. 2023; 24(23):16671. https://doi.org/10.3390/ijms242316671
Chicago/Turabian StyleLahlil, Rachid, Anne Aries, Maurice Scrofani, Céline Zanetti, Desline Hennequin, and Bernard Drénou. 2023. "Stem Cell Responsiveness to Imatinib in Chronic Myeloid Leukemia" International Journal of Molecular Sciences 24, no. 23: 16671. https://doi.org/10.3390/ijms242316671