Dietary Potassium Supplementation Reduces Chronic Kidney Lesions Independent of Blood Pressure in Deoxycorticosterone-Acetate and High Sodium Chloride-Treated Mice
Abstract
:1. Introduction
2. Results
2.1. DOCA/Salt-Induced Renal Lesion and Proinflammatory and Fibrotic Marker Genes in the Absence of Hypertension
2.2. Dietary Potassium Supplementation Reduces Renal Lesion and Proinflammatory and Fibrotic Marker Gene Expressions in DOCA/Salt Normotensive Mice
3. Discussion
4. Materials and Methods
4.1. Mice and Experimental Protocol
4.2. Blood Pressure, Serum and Urinary Electrolytes, Creatinine, and Protein
4.3. Renal Pathology
4.4. Renal Proinflammatory Marker Genes
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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TAP | DOCS | DOCS + KCl | ||||||
---|---|---|---|---|---|---|---|---|
5 Weeks | 8 Weeks | 11 Weeks | 5 Weeks | 8 Weeks | 11 Weeks | 8 + 3 Weeks | 11 + 6 Weeks | |
Number of mice | 9 | 12 | 12 | 10 | 9 | 10 | 11 | 12 |
Body weight (g) | 27 ± 0.4 | 28 ± 0.4 | 28 ± 0.3 | 28 ± 0.5 | 29 ± 0.3 | 29 ± 0.4 | 29 ± 0.4 | 29 ± 0.4 |
MBP (mmHg) | 113 ± 2 | 114 ± 2 | 114 ± 2 | 112 ± 2 | 110 ± 2 | 109 ± 3 | 109 ± 3 | 112 ± 3 |
Heart rate (beats/min) | 604 ± 20 | 601 ± 12 | 591 ± 15 | 600 ± 6 | 585 ± 8 | 578 ± 12 | 596 ± 10 | 577 ± 12 |
Serum Na+ (mmol/L) | 150 ± 1 | 151 ± 1 | 152 ± 1 | 153 ± 1 | 154 ± 1 | 154 ± 1 | 154 ± 1 | 154 ± 1 |
Kidney weight (mg) | 204 ± 4 | 236 ± 9 | 253 ± 6 | 357 ± 8 *** | 376 ± 9 *** | 399 ± 12 *** | 350 ± 6 † | 350 ± 4 †† |
UNa/Creat | 57 ± 4 | 54 ± 5 | 73 ± 6 | 289 ± 56 *** | 366 ± 5 *** | 389 ± 44 *** | 250 ± 44 | 444 ± 86 |
UK/Creat | 50 ± 2 | 55 ± 4 | 62 ± 4 | 56 ± 6 | 68 ± 3 | 62 ± 4 | 141 ± 14 ††† | 204 ± 29 †† |
UProt/Creat (g/mmol) | 0.8 ± 0.1 | 0.7 ± 0.03 | 0.7 ± 0.1 | 1.7 ± 0.2 ** | 1.1 ± 0.1 ** | 1.1 ± 0.1 ** | 0.8 ± 0.1 | 0.5 ± 0.1 † |
TAP | DOCS | DOCS+KCl | ||||
---|---|---|---|---|---|---|
8 weeks | 11 weeks | 8 Weeks | 11 Weeks | 8 + 3 Weeks | 11 + 6 Weeks | |
Number of mice | 9 | 10 | 11 | 8 | 9 | 12 |
Vascular wall thickness | none | none | yes | yes | focal | focal |
Tubular hypertrophy | none | none | focal | yes | focal or no | focal or yes |
Gene | Sense Primer (5′-3′) | Antisense Primer (5′-3′) |
---|---|---|
PAI-1 | GACTGGGTGGAAAGGCATAC | GCGTGTCAGCTCGTCTACAG |
Fibronectin | TCCTGCCTGGGACAGAATAC | TGAATGAGTTGGCGGTGATA |
Collagen type I | GATGGATTCCCGTTCGAGTA | AGGCCTCGGTGGACATTAG |
Collagen type III | TTGGAATTGCAGGGCTAACT | AGGACCACGTTCCCCATTAT |
IP-10 | CCCACGTGTTGAGATCATTG | CACTGGGTAAAGGGGAGTGA |
MCP-1 | CCCACTCACCTGCTGCTACT | GCTGCTGGTGATCCTCTTGTA |
TGF-β | CTGCTGACCCCCACTGATAC | GCTGAATCGAAAGCCCTGTA |
TNF-α | CGTCAGCCGATTTGCTATCT | CGGACTCCGCAAAGTCTAAG |
Osteopontin | TGCACCCAGATCCTATAGCC | CTCCATCGTCATCATCATCG |
P65 | GAGCCCATGGAGTTCCAGTA | TCGGGTAGGCACAGCAATAC |
ICAM | GGGGAACCCATCTCCTAAGA | AGGCATGGCACACGTATGTA |
ANP | ATGCTGGCAGCTAGGAGACA | AGGCCAAGACGAGGAAGAAG |
α-skeletal actin | GGACCTGTACGCCAACAACG | AGCCACCGATCCACACTGAG |
18S | CTCAACACGGGAAACCTCAC | AGACAAATCGCTCCACCAAC |
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Wang, Q.; Schäfer, S.C.; Haefliger, J.-A.; Maillard, M.P.; Alonso, F. Dietary Potassium Supplementation Reduces Chronic Kidney Lesions Independent of Blood Pressure in Deoxycorticosterone-Acetate and High Sodium Chloride-Treated Mice. Int. J. Mol. Sci. 2023, 24, 16858. https://doi.org/10.3390/ijms242316858
Wang Q, Schäfer SC, Haefliger J-A, Maillard MP, Alonso F. Dietary Potassium Supplementation Reduces Chronic Kidney Lesions Independent of Blood Pressure in Deoxycorticosterone-Acetate and High Sodium Chloride-Treated Mice. International Journal of Molecular Sciences. 2023; 24(23):16858. https://doi.org/10.3390/ijms242316858
Chicago/Turabian StyleWang, Qing, Stephan C. Schäfer, Jacques-Antoine Haefliger, Marc P. Maillard, and Florian Alonso. 2023. "Dietary Potassium Supplementation Reduces Chronic Kidney Lesions Independent of Blood Pressure in Deoxycorticosterone-Acetate and High Sodium Chloride-Treated Mice" International Journal of Molecular Sciences 24, no. 23: 16858. https://doi.org/10.3390/ijms242316858