Characterization of the Novel Leaderless Bacteriocin, Bawcin, from Bacillus wiedmannii
Abstract
:1. Introduction
2. Results and Discussion
2.1. Bawcin Identification and Biosynthetic Gene Cluster
2.2. Antimicrobial Activity of Bawcin
2.3. pH and Temperature Stability of Bawcin
2.4. Protease Stability of Bawcin
2.5. Structural Analysis of Bawcin
3. Materials and Methods
3.1. Bawcin and Bacterial Strains
3.2. Antimicrobial Activity Testing
3.3. pH Stability Testing
3.4. Temperature Stability Testing
3.5. Protease Sensitivity Testing
3.6. Sequence and Structural Analysis
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Protein | Size (aa) | TM | Identity |
---|---|---|---|
BawA | 48 | 0 | Bawcin (bacteriocin) |
BawB | 94 | 3 | Hypothetical protein |
BawC | 98 | 0 | DUF2089 family protein |
BawD | 390 | 1 | HlyD family efflux transporter |
BawE | 226 | 0 | ABC transporter ATP-binding protein |
BawF | 399 | 5 | ABC transporter permease |
BawG | 223 | 5 | Yip1 family protein |
Indicator Organism | MIC (µM) | Heteroresistance (µM) |
---|---|---|
Micrococcus luteus (Ward’s 85W 0966) | 4 | 4 |
Lactococcus lactis ssp. lactis (Ward’s 85W 1774) | 4 | 4 |
Lactobacillus delbruekii ssp. bulgaricus (VWR 470179-126) | 8 | 8 |
Bacillus subtilis (Ward’s 85W 1650) | 8 | 8, 16 |
Listeria monocytogenes ATCC 19115 | 8 | 8, 16 |
Lactobacillus casei (Ward’s 85W 1682) | 8 | 8–32 |
Bacillus cereus (Ward’s 85W 0200) | 16 | 16–64 |
Staphylococcus aureus ATCC 6538 | 16 | 16–64 |
Enterococcus faecalis (Ward’s 85W 1100) | 32 | 32–128 |
Staphylococcus epidermidis (Ward’s 85W 1747) | - | - |
Streptococcus pyogenes (Ward’s 85W 1180) | - | - |
Geobacillus stearothermophilus 1010 | - | - |
Pseudomonas aeruginosa (Ward’s 85W 1173) | - | - |
Salmonella enterica (Ward’s 85W 1710) | - | - |
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Budhwani, Z.; Buragina, J.T.; Lang, J.; Acedo, J.Z. Characterization of the Novel Leaderless Bacteriocin, Bawcin, from Bacillus wiedmannii. Int. J. Mol. Sci. 2023, 24, 16965. https://doi.org/10.3390/ijms242316965
Budhwani Z, Buragina JT, Lang J, Acedo JZ. Characterization of the Novel Leaderless Bacteriocin, Bawcin, from Bacillus wiedmannii. International Journal of Molecular Sciences. 2023; 24(23):16965. https://doi.org/10.3390/ijms242316965
Chicago/Turabian StyleBudhwani, Zafina, Jenna T. Buragina, Jen Lang, and Jeella Z. Acedo. 2023. "Characterization of the Novel Leaderless Bacteriocin, Bawcin, from Bacillus wiedmannii" International Journal of Molecular Sciences 24, no. 23: 16965. https://doi.org/10.3390/ijms242316965