Benzodiazepine Delorazepam Induces Locomotory Hyperactivity and Alterations in Pedal Mucus Texture in the Freshwater Gastropod Planorbarius corneus
Abstract
:1. Introduction
2. Results
2.1. Effects of Delorazepam on Locomotor Activity
2.2. Effects of Delorazepam on Feeding Behavior Response
2.3. Effects of Delorazepam on Mucus Texture
2.4. Pedal Glands and Role in Mucus Release
2.5. Characterization of Mucus Glycoconjugates
2.5.1. Pas and Alcian Staining
2.5.2. Staining with FITC-Lectins
2.6. Effects of DLZ on Foot Protein Pattern
3. Discussion
4. Materials and Methods
4.1. Animal Maintenance and Treatment
4.2. Thigmotactic Response (TR)
4.3. Food Search Test
4.4. Video Analysis and Speed Determination
4.5. Mucus Collection
4.6. Histological Analysis
4.7. Protein Analysis by SDS-PAGE
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Fogliano, C.; Carotenuto, R.; Agnisola, C.; Simoniello, P.; Karam, M.; Manfredonia, C.; Avallone, B.; Motta, C.M. Benzodiazepine Delorazepam Induces Locomotory Hyperactivity and Alterations in Pedal Mucus Texture in the Freshwater Gastropod Planorbarius corneus. Int. J. Mol. Sci. 2023, 24, 17070. https://doi.org/10.3390/ijms242317070
Fogliano C, Carotenuto R, Agnisola C, Simoniello P, Karam M, Manfredonia C, Avallone B, Motta CM. Benzodiazepine Delorazepam Induces Locomotory Hyperactivity and Alterations in Pedal Mucus Texture in the Freshwater Gastropod Planorbarius corneus. International Journal of Molecular Sciences. 2023; 24(23):17070. https://doi.org/10.3390/ijms242317070
Chicago/Turabian StyleFogliano, Chiara, Rosa Carotenuto, Claudio Agnisola, Palma Simoniello, Myriam Karam, Claudia Manfredonia, Bice Avallone, and Chiara Maria Motta. 2023. "Benzodiazepine Delorazepam Induces Locomotory Hyperactivity and Alterations in Pedal Mucus Texture in the Freshwater Gastropod Planorbarius corneus" International Journal of Molecular Sciences 24, no. 23: 17070. https://doi.org/10.3390/ijms242317070