Acid-Sensing Ion Channels’ Immunoreactivity in Nerve Profiles and Glomus Cells of the Human Carotid Body
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Immunohistochemistry
4.3. Double Immunofluorescence
4.4. Quantitative Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Glomus I Cell | Nerves | |
---|---|---|
ASIC1 | 43.2 ± 3.6 | 2.6 ± 0.3 |
ASIC2 | 61.1 ± 2.8 | 1.9 ± 0.2 |
ASIC3 | 9.7 ± 2.4 | 2.3 ± 0.3 |
ASIC4 | 2.4 ± 2.1 | 2.3 ± 0.9 |
Petrosal Ganglion | Cervical Superior Sympathetic Ganglion | |
---|---|---|
ASIC1 | 96.1 ± 9.2 | 100 |
ASIC2 | 98.6 ± 2.3 | 96.5 ± 1.1 |
ASIC3 | 96.4 ± 7.1 | 100 |
ASIC4 | 94.9 ± 5.2 | 98.1 ± 0.8 |
Antigen | Origin | Dilution | Supplier |
---|---|---|---|
ASIC1 | Rabbit | 1:200 | Abcam 1 |
ASIC2 | Rabbit | 1:200 | LifeSpan 2 |
ASIC3 | Rabbit | 1:100 | Abcam 1 |
ASIC4 | Rabbit | 1:100 | LifeSpan 2 |
NSE (clone BBS/NC/VI-H14) | Mouse | 1:500 | Dako 3 |
NFP (clone 2F11) | Mouse | 1:200 | Roche 4 |
Synaptophysin (clone 27G12) | Mouse | Prediluted | Leica Biosystems 5 |
S100P | Mouse | 1:500 | Dako 3 |
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Martínez-Barbero, G.; García-Mesa, Y.; Cobo, R.; Cuendias, P.; Martín-Biedma, B.; García-Suárez, O.; Feito, J.; Cobo, T.; Vega, J.A. Acid-Sensing Ion Channels’ Immunoreactivity in Nerve Profiles and Glomus Cells of the Human Carotid Body. Int. J. Mol. Sci. 2023, 24, 17161. https://doi.org/10.3390/ijms242417161
Martínez-Barbero G, García-Mesa Y, Cobo R, Cuendias P, Martín-Biedma B, García-Suárez O, Feito J, Cobo T, Vega JA. Acid-Sensing Ion Channels’ Immunoreactivity in Nerve Profiles and Glomus Cells of the Human Carotid Body. International Journal of Molecular Sciences. 2023; 24(24):17161. https://doi.org/10.3390/ijms242417161
Chicago/Turabian StyleMartínez-Barbero, Graciela, Yolanda García-Mesa, Ramón Cobo, Patricia Cuendias, Benjamín Martín-Biedma, Olivia García-Suárez, Jorge Feito, Teresa Cobo, and José A. Vega. 2023. "Acid-Sensing Ion Channels’ Immunoreactivity in Nerve Profiles and Glomus Cells of the Human Carotid Body" International Journal of Molecular Sciences 24, no. 24: 17161. https://doi.org/10.3390/ijms242417161