Genome-Wide Identification, Characterization, and Expression Analysis of the Copper-Containing Amine Oxidase Gene Family in Mangrove Kandelia obovata
Abstract
:1. Introduction
2. Results
2.1. Identification of CuAO Family Members in Kandelia obovata
2.2. Diversity within the CuAO Family in Relation to Domain and Three-Dimensional Structure
2.3. CuAO Protein Phylogenetic Relationships
2.4. Investigation of the Genetic Structure and Conserved Features of CuAO Genes
2.5. Cis-Regulatory Elements in the Promoters of Six CuAO Genes
2.6. Duplication Analysis of CuAO Gene Family
2.7. Expression Analysis of KoCuAO Genes under Copper Treatment
3. Discussion
4. Materials and Methods
4.1. Characterization and Identification of the CuAO Genes in Kandelia obovata
4.2. Chromosomal Distribution
4.3. Phylogenetic Tree Construction
4.4. Gene Structure and Significant Motif Analyses
4.5. Duplication Analysis
4.6. Cis-Regulatory Elements (CREs)
4.7. Three-Dimensional Structure and Subcellular Localization
4.8. Plant Material and Environmental Conditions
4.9. Quantitative Real-Time PCR Assays
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Gene ID | Location | AA 1 | Chains 2 | MW 3/kDd | pI 4 | GRAVY 5 | Subcellular (WoLF PSORT) | Subcellular (Plant-Ploc) |
---|---|---|---|---|---|---|---|---|---|
KoCuAO1 | geneMaker00012803 | Chr04 2370575-2373822 | 159 | + | 17.09 | 9.72 | 0.309 | Chloroplast | Cytoplasm |
KoCuAO2 | geneMaker00012751 | Chr04 2377019-2381467 | 106 | - | 11.18 | 8.71 | 0.498 | Cytoskeleton | Cytoplasm |
KoCuAO3 | geneMaker00008448 | Chr09 2334407-2341281 | 145 | - | 15.64 | 6.82 | 0.566 | Peroxisome | Chloroplast |
KoCuAO4 | geneMaker00006577 | Chr13 1070360-1075782 | 133 | - | 14.78 | 8.82 | 0.805 | Peroxisome | Chloroplast |
KoCuAO5 | geneMaker00006577 | Chr15 1433543-1437433 | 133 | - | 14.78 | 8.82 | 0.805 | Peroxisome | Chloroplast |
KoCuAO6 | geneMaker00006577 | Chr15 1441486-1445331 | 133 | - | 14.78 | 8.82 | 0.805 | Vacuole | Chloroplast |
Name | Method | Ka | Ks | Ka/Ks | Divergence-Time (MYA) | Duplicated Type |
---|---|---|---|---|---|---|
KoCuAO1 and KoCuAO2 | MS | 0.97 | 1.13 | 0.86 | 37.56 | tandem |
KoCuAO5 and KoCuAO6 | MS | 0.99 | 1.03 | 0.97 | 34.17 | tandem |
KoCuAO3 and KoCuAO4 | MS | 1.00 | 1.01 | 0.99 | 33.67 | segmental |
Gene Name | Primer Name | Sequence (5’-3’) | Length | Tm | GC% | Product Length |
---|---|---|---|---|---|---|
KoCuAO1 | 1-F | GTCAACCACAGCCCTACCTC | 20 | 60.04 | 60 | 165 |
1-R | GTGGCTGCTGTTTGCTCTTC | 20 | 60.04 | 55 | ||
KoCuAO2 | 2-F | TCAGAACCCACCATCCAAGC | 20 | 59.96 | 55 | 223 |
2-R | TGCCTCTGTTTTCAGCCGAT | 20 | 59.96 | 50 | ||
KoCuAO3 | 3-F | TGTCGCATCTCAACGAAGCA | 20 | 60.32 | 50 | 143 |
3-F | CAAGTCCCCAAGGCTGCATA | 20 | 60.03 | 55 | ||
KoCuAO4 | 4-F | GTGTCTGTTTGCACGAAGAGG | 22 | 59.4 | 54.5 | 193 |
4-R | TTCACCTCAACAACTGCCAT | 22 | 59.5 | 59.1 | ||
KoCuAO5 | 5-F | CACTCGTTGTCACTGGACGA | 20 | 59.97 | 55 | 92 |
5-R | CCGATCACTTGGGCTTTCCT | 20 | 60.04 | 55 | ||
KoCuAO6 | 6-F | TCACACGGACTCGTTCACAG | 20 | 59.97 | 55 | 157 |
6-R | AGACAATCGCTGCACTCACA | 20 | 59.97 | 50 |
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Hussain, Q.; Ye, T.; Shang, C.; Li, S.; Nkoh, J.N.; Li, W.; Hu, Z. Genome-Wide Identification, Characterization, and Expression Analysis of the Copper-Containing Amine Oxidase Gene Family in Mangrove Kandelia obovata. Int. J. Mol. Sci. 2023, 24, 17312. https://doi.org/10.3390/ijms242417312
Hussain Q, Ye T, Shang C, Li S, Nkoh JN, Li W, Hu Z. Genome-Wide Identification, Characterization, and Expression Analysis of the Copper-Containing Amine Oxidase Gene Family in Mangrove Kandelia obovata. International Journal of Molecular Sciences. 2023; 24(24):17312. https://doi.org/10.3390/ijms242417312
Chicago/Turabian StyleHussain, Quaid, Ting Ye, Chenjing Shang, Sihui Li, Jackson Nkoh Nkoh, Wenyi Li, and Zhangli Hu. 2023. "Genome-Wide Identification, Characterization, and Expression Analysis of the Copper-Containing Amine Oxidase Gene Family in Mangrove Kandelia obovata" International Journal of Molecular Sciences 24, no. 24: 17312. https://doi.org/10.3390/ijms242417312