Molecular Characterization of Local Walnut (Juglans regia) Genotypes in the North-East Parnon Mountain Region of Greece
Abstract
:1. Introduction
2. Results
2.1. Polymorphism of SSR Primer Pairs
2.2. Population Structure Analysis
2.3. Principal Coordinate Analysis
2.4. UPGMA Cluster Analysis
2.5. Genetic Diversity Analysis
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Molecular Analysis Using SSR Markers
4.3. Molecular Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer | Na | Ne | Ho | He | I | PIC | PI |
---|---|---|---|---|---|---|---|
WGA009 | 9.50 | 7.78 | 0.54 | 0.84 | 2.03 | 0.94 | 0.0073 |
WGA321 | 9.50 | 8.07 | 0.62 | 0.85 | 2.06 | 0.95 | 0.0059 |
WGA349 | 9.17 | 7.50 | 0.57 | 0.84 | 2.04 | 0.94 | 0.0071 |
WGA069 | 9.83 | 8.46 | 0.60 | 0.87 | 2.17 | 0.95 | 0.0057 |
WGA118 | 6.50 | 5.94 | 0.03 | 0.76 | 1.70 | 0.93 | 0.0102 |
WGA202 | 6.67 | 6.20 | 0.00 | 0.78 | 1.73 | 0.94 | 0.0059 |
WGA276 | 9.17 | 7.33 | 0.54 | 0.83 | 2.00 | 0.94 | 0.0073 |
WGA001 | 12.83 | 11.27 | 0.84 | 0.88 | 2.37 | 0.97 | 0.0019 |
WGA376 | 10.67 | 9.23 | 0.53 | 0.87 | 2.20 | 0.96 | 0.0035 |
Mean | 9.3 | 7.97 | 0.47 | 0.84 | 2.03 | 0.94 | 0.0061 |
No. | Local Walnut Selection | Latitude | Longitude | Altitude |
---|---|---|---|---|
1 | GR-PARNON-01 | 37°15′13.8″ N | 22°33′14.2″ E | 1031 |
2 | GR-PARNON-02 | 37°15′13.3″ N | 22°33′14.2″ E | 1011 |
3 | GR-PARNON-03 | 37°14′27.1″ N | 22°33′26.5″ E | 1079 |
4 | GR-PARNON-04 | 37°14′18.1″ N | 22°33′25.0″ E | 1045 |
5 | GR-PARNON-05 | 37°14′36.3″ N | 22°30′22.4″ E | 720 |
6 | GR-PARNON-06 | 37°14′42.4″ N | 22°30′15.5″ E | 755 |
7 | GR-PARNON-07 | 37°14′39.6″ N | 22°30′35.4″ E | 730 |
8 | GR-PARNON-08 | 37°14′54.1″ N | 22°34′05.3″ E | 1062 |
9 | GR-PARNON-09 | 37°14′55.8″ N | 22°34′34.6″ E | 1224 |
10 | GR-PARNON-10 | 37°14′55.7″ N | 22°34′37.3″ E | 1247 |
11 | GR-PARNON-11 | 37°14′28.5″ N | 22°34′31.6″ E | 1297 |
12 | GR-PARNON-12 | 37°14′27.3″ N | 22°34′42.7″ E | 1280 |
13 | GR-PARNON-13 | 37°14′22.9″ N | 22°34′36.6″ E | 1278 |
14 | GR-PARNON-14 | 37°14′35.0″ N | 22°34′20.3″ E | 1212 |
15 | GR-PARNON-15 | 37°16′22.1″ N | 22°31′32.1″ E | 1020 |
16 | GR-PARNON-16 | 37°15′49.1″ N | 22°31′58.9″ E | 950 |
17 | GR-PARNON-17 | 37°15′47.4″ N | 22°32′02.3″ E | 980 |
18 | GR-PARNON-18 | 37°15′44.2″ N | 22°31′57.6″ E | 940 |
19 | GR-PARNON-19 | 37°15′55.4″ N | 22°32′50.1″ E | 1135 |
20 | GR-PARNON-20 | 37°15′55.0″ N | 22°32′51.0″ E | 1105 |
21 | GR-PARNON-21 | 37°15′58.4″ N | 22°33′10.5″ E | 1100 |
22 | GR-PARNON-22 | 37°14′47.2″ N | 22°32′30.1″ E | 865 |
23 | GR-PARNON-23 | 37°15′21.7″ N | 22°31′33.2″ E | 910 |
24 | GR-PARNON-24 | 37°15′19.5″ N | 22°31′32.7″ E | 878 |
25 | GR-PARNON-25 | 37°14′57.0″ N | 22°32′25.2″ E | 840 |
26 | GR-PARNON-26 | 37°15′57.6″ N | 22°32′58.4″ E | 1127 |
27 | GR-PARNON-27 | 37°16′08.6″ N | 22°31′53.3″ E | 1110 |
28 | GR-PARNON-28 | 37°14′20.3″ N | 22°33′04.1″ E | 921 |
29 | GR-PARNON-29 | 37°16′24.3″ N | 22°32′42.9″ E | 1279 |
30 | GR-PARNON-30 | 37°14′37.5″ N | 22°32′40.8″ E | 884 |
31 | GR-PARNON-31 | 37°16′09.3″ N | 22°32′19.1″ E | 1204 |
32 | GR-PARNON-32 | 37°16′15.1″ N | 22°32′06.1″ E | 1217 |
33 | GR-PARNON-33 | 37°16′17.8″ N | 22°32′08.3″ E | 1232 |
34 | GR-PARNON-34 | 37°14′40.6″ N | 22°33′09.9″ E | 985 |
35 | GR-PARNON-35 | 37°14′38.7″ N | 22°33′09.5″ E | 1001 |
36 | GR-PARNON-36 | 37°14′38.6″ N | 22°33′05.4″ E | 950 |
37 | GR-PARNON-37 | 37°14′45.9″ N | 22°34′07.8″ E | 1078 |
38 | GR-PARNON-38 | 37°14′46.6″ N | 22°34′51.7″ E | 1290 |
39 | GR-PARNON-39 | 37°14′54.1″ N | 22°34′05.4″ E | 1061 |
40 | GR-PARNON-40 | 37°14′39.7″ N | 22°33′09.5″ E | 803 |
41 | GR-PARNON-41 | 37°15′01.2″ N | 22°34′34.9″ E | 1287 |
42 | GR-PARNON-42 | 37°17′18.0″ N | 22°29′31.0″ E | 905 |
43 | GR-PARNON-43 | 37°15′30.4″ N | 22°32′37.7″ E | 1041 |
44 | GR-PARNON-44 | 37°16′37.9″ N | 22°31′04.2″ E | 1053 |
45 | GR-PARNON-45 | 37°17′21.8″ N | 22°30′17.7″ E | 880 |
46 | GR-PARNON-46 | 37°16′30.7″ N | 22°31′37.3″ E | 1052 |
47 | GR-PARNON-47 | 37°16′06.8″ N | 22°31′48.9″ E | 1036 |
Primer | Primer Sequence (5′–3′) | Repeat Motif | Annealing Temperature (°C) | Product Size Range (bp) |
---|---|---|---|---|
WGA009 | F CATCAAAGCAAGCAATGGG | (GA)16 | 51 | 231–245 |
R CCATTGCTCTGTGATTGGG | ||||
WGA321 | F TCCAATCGAAACTCCAAAGG | (GA)14 | 51 | 223–245 |
R TGTCCAAAGACGATGATGGA | ||||
WGA349 | F GTGGCGAAAGTTTATTTTTTGC | (CT)14 | 52 | 262–274 |
R ACAAATGCACAGCAGCAAAC | ||||
WGA069 | F TTAGTTAGCAAACCCACCCG | (GA)4ATATAA(GA)1 | 54 | 160–182 |
R AGATGCACAGACCAACCCTC | ||||
WGA118 | F TGTGCTCTGATCTGCCTCC | (GA)18(GT)11 | 54 | 186–200 |
R GGGTGGGTGAAAAGTAGCAA | ||||
WGA202 | F CCCATCTACCGTTGCACTTT | (GA)11 | 54 | 259–295 |
R GCTGGTGGTTCTATCATGGG | ||||
WGA276 | F CTCACTTTCTCGGCTCTTCC | (GA)14 | 55 | 168–194 |
R GGTCTTATGTGGGCAGTCGT | ||||
WGA001 | F ATTGGAAGGGAAGGGAAATG | (GA)5GCA(GA)3GCA(GA)3 | 52 | 180–192 |
R CGCGCACATACGTAAATCAC | ||||
WGA376 | F GCCCTCAAAGTGATGAACGT | (AG)2AA(AG)6 | 54 | 230–265 |
R TCATCCATATTTACCCCTTTCG |
Groups of Samples | Altitude Range |
---|---|
1 | 720–755 m |
2 | 803–884 m |
3 | 905–985 m |
4 | 1001–1079 m |
5 | 1100–1135 m |
6 | 1204–1297 m |
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Manthos, I.; Sotiropoulos, T.; Karapetsi, L.; Ganopoulos, I.; Pratsinakis, E.D.; Maloupa, E.; Madesis, P. Molecular Characterization of Local Walnut (Juglans regia) Genotypes in the North-East Parnon Mountain Region of Greece. Int. J. Mol. Sci. 2023, 24, 17230. https://doi.org/10.3390/ijms242417230
Manthos I, Sotiropoulos T, Karapetsi L, Ganopoulos I, Pratsinakis ED, Maloupa E, Madesis P. Molecular Characterization of Local Walnut (Juglans regia) Genotypes in the North-East Parnon Mountain Region of Greece. International Journal of Molecular Sciences. 2023; 24(24):17230. https://doi.org/10.3390/ijms242417230
Chicago/Turabian StyleManthos, Ioannis, Thomas Sotiropoulos, Lefkothea Karapetsi, Ioannis Ganopoulos, Emmanouil D. Pratsinakis, Eleni Maloupa, and Panagiotis Madesis. 2023. "Molecular Characterization of Local Walnut (Juglans regia) Genotypes in the North-East Parnon Mountain Region of Greece" International Journal of Molecular Sciences 24, no. 24: 17230. https://doi.org/10.3390/ijms242417230