Antiproliferative Activity of Antibiotics through DNA Binding Mechanism: Evaluation and Molecular Docking Studies
Abstract
:1. Introduction
2. Results and Discussion
2.1. General Aspects
2.2. Ex Vivo Studies
DNA Binding Studies
2.3. In Silico Studies
Computational Studies—Molecular Docking
2.4. In Vitro Studies
2.4.1. Anti-Proliferative Activity
2.4.2. Cell Cycle Arrest Study
3. Conclusions
4. Materials and Methods
4.1. Chemicals and Instruments
4.2. DNA Binding Studies
4.2.1. Ultraviolet−Visible Studies
4.2.2. Viscosity Measurements
4.3. Molecular Docking
4.4. Sulforhodamine B (SRB) Assay
4.5. Cell Cycle Arrest
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Antibiotic | Kb (M−1) | Hyper-/Hypochromic Effect | H (%) |
---|---|---|---|
CIP·HCl | (2.8 ± 0.6) × 104 | Hyperchromism | 3.3 |
PEN·Na | (0.4 ± 0.1) × 104 | Hypochromism | 1.4 |
TC·HCl | (6.9 ± 0.3) × 104 | Hyperchromism | 8.5 |
Antibiotic | Binding Energy (kcal/mol) | DNA Strand | Nucleotide | Nucleotide’s Atom | Atoms in Compound | Distance (Å) |
---|---|---|---|---|---|---|
CIP·HCl | −5.41 | B | dG14 | H7 | O2 | 2.242 |
A | dT8 | O2 | H41 | 1.892 | ||
PEN·Na | −6.13 | A | dG10 | H21 | O2 | 1.669 |
B | dG16 | H22 | O5 | 2.205 | ||
TC·HCl | −6.11 | A | dA6 | O3 | H51 | 2.038 |
B | dC21 | O2 | H42 | 2.009 | ||
A | dA6 | O3 | H56 | 1.809 | ||
DOX | −9.36 | A | dG10 | H3 | O7 | 1.872 |
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Magklaras, A.-D.C.; Banti, C.N.; Hadjikakou, S.K. Antiproliferative Activity of Antibiotics through DNA Binding Mechanism: Evaluation and Molecular Docking Studies. Int. J. Mol. Sci. 2023, 24, 2563. https://doi.org/10.3390/ijms24032563
Magklaras A-DC, Banti CN, Hadjikakou SK. Antiproliferative Activity of Antibiotics through DNA Binding Mechanism: Evaluation and Molecular Docking Studies. International Journal of Molecular Sciences. 2023; 24(3):2563. https://doi.org/10.3390/ijms24032563
Chicago/Turabian StyleMagklaras, Alexandros-Dimitrios C., Christina N. Banti, and Sotiris K. Hadjikakou. 2023. "Antiproliferative Activity of Antibiotics through DNA Binding Mechanism: Evaluation and Molecular Docking Studies" International Journal of Molecular Sciences 24, no. 3: 2563. https://doi.org/10.3390/ijms24032563