Monoglyceride Lipase Deficiency Is Associated with Altered Thrombogenesis in Mice
Abstract
:1. Introduction
2. Results
2.1. Unaltered Morphology and Spreading in Mgl−/− Platelets
2.2. Increased Mitochondrial Respiration in Mgl−/− Platelets
2.3. Loss of MGL Affects Thrombus Formation In Vitro
2.4. Reduced Hemostatic Function in Mgl−/− but Not in platMgl−/− Mice
2.5. Loss of MGL Affects Thrombus Formation In Vivo
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Platelet Isolation and Purification
4.3. Transmission and Scanning Electron Microscopy
4.4. Targeted Lipidomic Analysis
4.5. Flow Cytometric Analyses of P-Selectin and αIIbβ3 Activation
4.6. Platelet Aggregation, Tail Bleeding, and Hemoglobin Assays
4.7. Mitochondrial Respiration Measurement
4.8. Thrombus Formation In Vitro
4.9. Thrombus Formation In Vivo
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Goeritzer, M.; Kuentzel, K.B.; Beck, S.; Korbelius, M.; Rainer, S.; Bradić, I.; Kolb, D.; Mussbacher, M.; Schrottmaier, W.C.; Assinger, A.; et al. Monoglyceride Lipase Deficiency Is Associated with Altered Thrombogenesis in Mice. Int. J. Mol. Sci. 2023, 24, 3116. https://doi.org/10.3390/ijms24043116
Goeritzer M, Kuentzel KB, Beck S, Korbelius M, Rainer S, Bradić I, Kolb D, Mussbacher M, Schrottmaier WC, Assinger A, et al. Monoglyceride Lipase Deficiency Is Associated with Altered Thrombogenesis in Mice. International Journal of Molecular Sciences. 2023; 24(4):3116. https://doi.org/10.3390/ijms24043116
Chicago/Turabian StyleGoeritzer, Madeleine, Katharina B. Kuentzel, Sarah Beck, Melanie Korbelius, Silvia Rainer, Ivan Bradić, Dagmar Kolb, Marion Mussbacher, Waltraud C. Schrottmaier, Alice Assinger, and et al. 2023. "Monoglyceride Lipase Deficiency Is Associated with Altered Thrombogenesis in Mice" International Journal of Molecular Sciences 24, no. 4: 3116. https://doi.org/10.3390/ijms24043116