Activation of Endothelial Large Conductance Potassium Channels Protects against TNF-α-Induced Inflammation
Abstract
:1. Introduction
2. Results
2.1. L-Type CaV Channels Regulate TNF-α-Induced Inflammatory Cytokine Secretion from Human Pulmonary Endothelial Cells
2.2. BK Channel Activation Decreases Inflammatory CCL-2 Secretion via Inhibition of Ca2+-Dependent JNK-1/2 and p38 Signaling Pathways
3. Discussion
4. Materials and Methods
4.1. Cells
4.1.1. Cell Culture Models and Cytokine Measurements
4.1.2. Em Measurements
4.1.3. Gene Expression
4.2. Prediction of Protein–Protein Interaction (PPI) Networks Using Functional Enrichment Analysis (STRING)
4.3. Experimental Validation of Predicted PPI Networks
4.4. Chemical/Drug-Protein Interaction Analysis (STITCH)
5. Significance Statement
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zyrianova, T.; Zou, K.; Lopez, B.; Liao, A.; Gu, C.; Olcese, R.; Schwingshackl, A. Activation of Endothelial Large Conductance Potassium Channels Protects against TNF-α-Induced Inflammation. Int. J. Mol. Sci. 2023, 24, 4087. https://doi.org/10.3390/ijms24044087
Zyrianova T, Zou K, Lopez B, Liao A, Gu C, Olcese R, Schwingshackl A. Activation of Endothelial Large Conductance Potassium Channels Protects against TNF-α-Induced Inflammation. International Journal of Molecular Sciences. 2023; 24(4):4087. https://doi.org/10.3390/ijms24044087
Chicago/Turabian StyleZyrianova, Tatiana, Kathlyn Zou, Benjamin Lopez, Andy Liao, Charles Gu, Riccardo Olcese, and Andreas Schwingshackl. 2023. "Activation of Endothelial Large Conductance Potassium Channels Protects against TNF-α-Induced Inflammation" International Journal of Molecular Sciences 24, no. 4: 4087. https://doi.org/10.3390/ijms24044087