miRNome and Proteome Profiling of Human Keratinocytes and Adipose Derived Stem Cells Proposed miRNA-Mediated Regulations of Epidermal Growth Factor and Interleukin 1-Alpha
Abstract
:1. Introduction
2. Results
2.1. Direct Co-Culture Can Enhance the Commitment of ADSCs towards the Epidermal Lineage
2.2. 378 miRNAs Differentially Expressed between Primary Keratinocytes and ADSCs
2.3. Differentially Expressed miRNAs–mRNAs Interactome and Enrichment Analysis of the Upregulated miRNA Targets in Keratinocytes Reveals 14 Pathways
2.4. Proteome Profiling and Integrated Analysis with Differentially Expressed miRNAs Highlights miRNA-Mediated Regulations
3. Discussion
4. Materials and Methods
4.1. Epidermal Differentiation Induction in Direct Co-Culture Model
4.2. Primary Cell Isolation
4.3. Human miRNome Profiling with Microarrays
4.4. Gene and miRNA Expression
4.5. DEmiRNAs-mRNA Interaction Network and Pathway Enrichment Analysis
4.6. Immunocytochemistry Staining
4.7. Proteome Profiling
4.8. miRNA-Mediated Gene and Protein Regulations (Integrated Analysis)
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Shahin, H.; Abdallah, S.; Das, J.; He, W.; El-Serafi, I.; Steinvall, I.; Sjöberg, F.; Elmasry, M.; El-Serafi, A.T. miRNome and Proteome Profiling of Human Keratinocytes and Adipose Derived Stem Cells Proposed miRNA-Mediated Regulations of Epidermal Growth Factor and Interleukin 1-Alpha. Int. J. Mol. Sci. 2023, 24, 4956. https://doi.org/10.3390/ijms24054956
Shahin H, Abdallah S, Das J, He W, El-Serafi I, Steinvall I, Sjöberg F, Elmasry M, El-Serafi AT. miRNome and Proteome Profiling of Human Keratinocytes and Adipose Derived Stem Cells Proposed miRNA-Mediated Regulations of Epidermal Growth Factor and Interleukin 1-Alpha. International Journal of Molecular Sciences. 2023; 24(5):4956. https://doi.org/10.3390/ijms24054956
Chicago/Turabian StyleShahin, Hady, Sallam Abdallah, Jyotirmoy Das, Weihai He, Ibrahim El-Serafi, Ingrid Steinvall, Folke Sjöberg, Moustafa Elmasry, and Ahmed T. El-Serafi. 2023. "miRNome and Proteome Profiling of Human Keratinocytes and Adipose Derived Stem Cells Proposed miRNA-Mediated Regulations of Epidermal Growth Factor and Interleukin 1-Alpha" International Journal of Molecular Sciences 24, no. 5: 4956. https://doi.org/10.3390/ijms24054956