In Vitro Characteristics of Canine Primary Tracheal Epithelial Cells Maintained at an Air–Liquid Interface Compared to In Vivo Morphology
Abstract
:1. Introduction
2. Results
2.1. In Vitro Epithelium Formation of Primary Canine Tracheal Epithelial Cells
2.2. Development of Barrier Function in Primary Canine Tracheal Epithelial Cells
2.3. In Vitro Ciliogenesis in Primary Canine Tracheal Epithelial Cells
2.4. In Vitro Differentiation of Goblet Cells Derived from Primary Tracheal Epithelial Cells
3. Discussion
4. Materials and Methods
4.1. Collection of Samples
4.2. Isolation and Maintenance of Canine Tracheal Epithelial Cell Cultures
4.3. Establishment of ALI Cultures
4.4. TEER Measurements
4.5. Histochemistry (H&E and Alcian Blue)
4.6. Immunohistochemistry and Immunofluorescence
4.7. Histological Evaluation of ALI Cultures
4.8. Scanning Electron Microscopy
4.9. Transmission Electron Microscopy
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Runft, S.; Färber, I.; Krüger, J.; Schöne, K.; Lehmbecker, A.; Baumgärtner, W. In Vitro Characteristics of Canine Primary Tracheal Epithelial Cells Maintained at an Air–Liquid Interface Compared to In Vivo Morphology. Int. J. Mol. Sci. 2023, 24, 4987. https://doi.org/10.3390/ijms24054987
Runft S, Färber I, Krüger J, Schöne K, Lehmbecker A, Baumgärtner W. In Vitro Characteristics of Canine Primary Tracheal Epithelial Cells Maintained at an Air–Liquid Interface Compared to In Vivo Morphology. International Journal of Molecular Sciences. 2023; 24(5):4987. https://doi.org/10.3390/ijms24054987
Chicago/Turabian StyleRunft, Sandra, Iris Färber, Johannes Krüger, Kerstin Schöne, Annika Lehmbecker, and Wolfgang Baumgärtner. 2023. "In Vitro Characteristics of Canine Primary Tracheal Epithelial Cells Maintained at an Air–Liquid Interface Compared to In Vivo Morphology" International Journal of Molecular Sciences 24, no. 5: 4987. https://doi.org/10.3390/ijms24054987