FT-IR Analysis of P. aeruginosa Bacteria Inactivation by Femtosecond IR Laser Radiation
Abstract
:1. Introduction
2. Results and Discussion
2.1. CFU Count
2.2. SEM and TEM Characterization
2.3. XRD Analysis
2.4. FT-IR Spectral Analysis
3. Materials and Methods
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Tyagi, L.; Sharma, G.P.; Verma, R.C.; Jain, S.K.; Murdia, L.K.; Mathur, S.M. Infrared heating in food processing: An overview. IJCS 2020, 8, 327–336. [Google Scholar] [CrossRef]
- Elliott, D.C.; Elliott, E.W. Biochemistry and Molecular Biology, 2nd ed.; Oxford University Press: Oxford, UK, 2001. [Google Scholar]
- Sawai, J.; Fujisawa, M.; Kokugan, T.; Shimizu, M.; Igarashi, H.; Hashimoto, A.; Kojima, H. Pasteurization of bacterial spores in liquid medium by far-infrared irradiation. J. Chem. Eng. Jpn. 1997, 30, 170–172. [Google Scholar] [CrossRef] [Green Version]
- Krishnamurthy, K.; Demirci, A.L.I.; Irudayaraj, J. Inactivation of Staphylococcus aureus by pulsed UV-light sterilization. J. Food Prot. 2004, 67, 1027–1030. [Google Scholar] [CrossRef]
- Hamanaka, D.; Uchino, T.; Furuse, N.; Han, W.; Tanaka, S.I. Effect of the wavelength of infrared heaters on the inactivation of bacterial spores at various water activities. Int. J. Food Microbiol. 2006, 108, 281–285. [Google Scholar] [CrossRef] [PubMed]
- Oduola, A.A.; Bowie, R.; Wilson, S.A.; Mohammadi Shad, Z.; Atungulu, G.G. Impacts of broadband and selected infrared wavelength treatments on inactivation of microbes on rough rice. J. Food Saf. 2020, 40, e12764. [Google Scholar] [CrossRef]
- Schoop, U.; Moritz, A.; Kluger, W.; Patruta, S.; Goharkhay, K.; Sperr, W.; Georgopoulos, A. The Er: YAG laser in endodontics: Results of an in vitro study. Lasers Surg. Med. Off. J. Am. Soc. Lasers Surg. Med. 2002, 30, 360–364. [Google Scholar] [CrossRef]
- Folwaczny, M.; Mehl, A.; Aggstaller, H.; Hickel, R. Antimicrobial effects of 2.94 μm Er: YAG laser radiation on root surfaces: An in vitro study. J. Clin. Periodontol. 2002, 29, 73–78. [Google Scholar] [CrossRef]
- Hashimoto, A.; Yamazaki, Y.; Shimizu, M.; Oshita, S.I. Drying characteristics of gelatinous materials irradiated by infrared radiation. Dry. Technol. 1994, 12, 1029–1052. [Google Scholar] [CrossRef]
- Pawar, S.B.; Pratape, V.M. Fundamentals of infrared heating and its application in drying of food materials: A review. J. Food Proc. Eng. 2017, 40, e12308. [Google Scholar] [CrossRef]
- Kompanets, V.O.; Kudryashov, S.I.; Totordava, E.R.; Shelygina, S.N.; Sokolova, V.V.; Saraeva, I.N.; Kovalev, M.S.; Ionin, A.A.; Chekalin, S.V. Femtosecond infrared laser spectroscopy of characteristic molecular vibrations in bacteria in the 6-µm spectral range. JETP Lett. 2021, 113, 365–369. [Google Scholar] [CrossRef]
- Kompanets, V.; Shelygina, S.; Tolordava, E.; Kudryashov, S.; Saraeva, I.; Rupasov, A.; Kovalev, M. Spectrally-selective mid-IR laser-induced inactivation of pathogenic bacteria. Biomed. Opt. Express 2021, 12, 6317–6325. [Google Scholar] [CrossRef]
- Rabiei, M.; Palevicius, A.; Dashti, A.; Nasiri, S.; Monshi, A.; Vilkauskas, A.; Janusas, G. Measurement Modulus of elasticity related to the atomic density of planes in unit cell of crystal lattices. Materials 2020, 13, 4380. [Google Scholar] [CrossRef]
- Nikaido, H.; Nakae, T. The outer membrane of Gram-negative bacteria. Adv. Microb. Physiol. 1980, 20, 163–250. [Google Scholar] [CrossRef]
- Tang, M.; McEwen, G.D.; Wu, Y.; Miller, C.D.; Zhou, A. Characterization and analysis of mycobacteria and Gram-negative bacteria and co-culture mixtures by Raman microspectroscopy, FTIR, and atomic force microscopy. Anal. Bioanal. Chem. 2013, 405, 1577–1591. [Google Scholar] [CrossRef] [PubMed]
- Davis, R.; Mauer, L.J. Fourier transform infrared FT-IR spectroscopy: A rapid tool for detection and analysis of foodborne pathogenic bacteria. Curr. Res. Technol. Educ. Top. Appl. Microbiol. Microb.Biotechnol. 2010, 2, 1582–1594. [Google Scholar]
- Melin, A.M.; Perromat, A.; Déléris, G. Pharmacologic application of Fourier transform IR spectroscopy: In vivo toxicity of carbon tetrachloride on rat liver. Biopolym. Orig. Res. Biomol. 2000, 53, 160–168. [Google Scholar] [CrossRef]
- Gorgulu, S.T.; Dogan, M.; Severcan, F. The characterization and differentiation of higher plants by Fourier transform infrared spectroscopy. Appl. Spectrosc. 2007, 61, 300–308. [Google Scholar] [CrossRef]
- Naumann, D. FT-infrared and FT-Raman spectroscopy in biomedical research. Appl. Spec. Rev. 2001, 36, 239–298. [Google Scholar] [CrossRef]
- Kamnev, A.A.; Tugarova, A.V.; Dyatlova, Y.A.; Tarantilis, P.A.; Grigoryeva, O.P.; Fainleib, A.M.; De Luca, S. Methodological effects in Fourier transform infrared FTIR spectroscopy: Implications for structural analyses of biomacromolecular samples. Spec. Act. Part A Mol. Biomol. Spec. 2018, 193, 558–564. [Google Scholar] [CrossRef]
- Quilès, F.; Humbert, F.; Delille, A. Analysis of changes in attenuated total reflection FTIR fingerprints of Pseudomonas fluorescens from planktonic state to nascent biofilm state. Spec. Act. Part A Mol. Biomol. Spec. 2010, 75, 610–616. [Google Scholar] [CrossRef]
- Zhu, L.; Qi, H.Y.; Kong, Y.; Yu, Y.W.; Xu, X.Y. Component analysis of extracellular polymeric substances during aerobic sludge granulation using FTIR and 3D-EEM technologies. Bioresour. Technol. 2012, 124, 455–459. [Google Scholar] [CrossRef] [PubMed]
- Zhao, X.; Lam, J.S. WaaP of Pseudomonas aeruginosa is a novel eukaryotic type protein-tyrosine kinase as well as a sugar kinase essential for the biosynthesis of core lipopolysaccharide. J. Biol. Chem. 2002, 277, 4722–4730. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jackson, M.; Sowa, M.G.; Mantsch, H.H. Infrared spectroscopy: A new frontier in medicine. Biophys. Chem. 1997, 68, 109–125. [Google Scholar] [CrossRef] [PubMed]
- Garip, S.; Gozen, A.C.; Severcan, F. Use of Fourier transform infrared spectroscopy for rapid comparative analysis of Bacillus and Micrococcus isolates. Food Chem. 2009, 113, 1301–1307. [Google Scholar] [CrossRef]
Exposure Time, min | P. aeruginosa, CFU/mL | |
---|---|---|
Control | - | 5·104 |
After 3.15 μm | 3 5 7 | 5·103 3·103 2·103 |
After 6.04 μm | 3 5 7 | 1·102 5·103 4·103 |
Functional Groups | Frequency (cm−1) Bandwidth (a.u.) Area (a.u.) | ||
---|---|---|---|
Control | 3.15 μm Exposure | 6.04 μm Exposure | |
PO2 str (sym) of nucleic acids and phospholipids | 1079.97 163.82 11.01 | 1079.97 123.37 6.76 | 1079.97 177.61 11.04 |
P=O str (asym) of phosphodiesters | 1240.04 52.95 1.88 | 1240.04 53.28 1.67 | 1240.04 53.35 1.34 |
COO¯ | 1403.97 48.13 4.13 | 1402.04 45.25 3.29 | 1402.04 47.21 2.75 |
C-H def of CH2 | 1450.25 32.97 1.33 | 1450.25 40.19 1.61 | 1450.25 40.21 1.32 |
Tyrosine O-H, C-C, C-H | 1517.75 38.78 2.13 | 1517.75 60.31 4.18 | 1517.75 64.62 3.28 |
Amide II N–H bend, C–N str of proteins | 1544.75 48.19 3.95 | 1544.75 52.94 3.50 | 1542.82 58.79 3.06 |
Amide I β-pleated sheets | 1637.32 51.26 7.21 | 1637.32 47.80 5.76 | 1637.32 48.61 4.59 |
Amide I α-helices | 1658.53 34.06 4.43 | 1658.53 34.86 4.42 | 1658.53 34.45 3.33 |
Amide I β-pleated sheets | 1681.67 29.08 2.84 | 1681.67 28.60 2.41 | 1681.67 27.87 1.82 |
C-H str (sym) of CH2 in fatty acids | 2852.29 32.79 0.43 | 2852.29 22.25 0.18 | 2852.29 11.11 0.06 |
C-H str (sym) of CH3 CH3 str (sym) of mainly proteins | 2873.51 27.65 0.51 | 2873.51 30.36 0.53 | 2875.43 69.43 1.27 |
C-H str (asym) in CH2 of mainly lipids | 2927.50 34.59 1.19 | 2927.50 36.56 1.20 | 2925.58 42.66 1.43 |
C-H str (asym) in CH3 of fatty acids | 2962.22 49.46 2.27 | 2962.22 46.98 1.85 | 2962.22 40.09 1.11 |
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Saraeva, I.; Tolordava, E.; Sheligyna, S.; Nastulyavichus, A.; Khmelnitskii, R.; Pokryshkin, N.; Khmelenin, D.; Kudryashov, S.; Ionin, A.; Akhmatkhanov, A. FT-IR Analysis of P. aeruginosa Bacteria Inactivation by Femtosecond IR Laser Radiation. Int. J. Mol. Sci. 2023, 24, 5119. https://doi.org/10.3390/ijms24065119
Saraeva I, Tolordava E, Sheligyna S, Nastulyavichus A, Khmelnitskii R, Pokryshkin N, Khmelenin D, Kudryashov S, Ionin A, Akhmatkhanov A. FT-IR Analysis of P. aeruginosa Bacteria Inactivation by Femtosecond IR Laser Radiation. International Journal of Molecular Sciences. 2023; 24(6):5119. https://doi.org/10.3390/ijms24065119
Chicago/Turabian StyleSaraeva, Irina, Eteri Tolordava, Svetlana Sheligyna, Alyona Nastulyavichus, Roman Khmelnitskii, Nikolay Pokryshkin, Dmitriy Khmelenin, Sergey Kudryashov, Andrey Ionin, and Andrey Akhmatkhanov. 2023. "FT-IR Analysis of P. aeruginosa Bacteria Inactivation by Femtosecond IR Laser Radiation" International Journal of Molecular Sciences 24, no. 6: 5119. https://doi.org/10.3390/ijms24065119