Interplay between Inter-Subunit Rotation of the Ribosome and Binding of Translational GTPases
Round 1
Reviewer 1 Report
Comments for author File: Comments.pdf
Author Response
We are glad that both reviewers found our manuscript interesting and worthy publishing in IJMS. We have fixed all the typos found by reviewers. Following their advice, we also added new Figure 5 that summarizes our findings.
We hope that our manuscript is now ready for publication.
Reviewer 2 Report
The current manuscript by Das, Adiletta and Ermolenko addresses a question of how the binding of ribosome GTPases LepA and EF-G affects the equilibrium between the none-rotated (NR) and (rotated) R conformations of the ribosome More specifically, using smFRET the authors study how the binding of translational GTPases affects intersubunit rotation of the ribosome. Undoubtedly, this topic is extremely important because it is directly related to deciphering of the mechanism of the ribosome working as a molecular maсhine. In the case of EF-G, a lot of data has been previously accumulated in the literature on its interaction with the ribosome during translocation So, the authors in fact convincingly and elegantly proved the widely accepted model by Rodnina et al. that required additional evidence. However, in the case of LepA, such data has not been previously obtained. Overall, this work is a very well done and presented as a very clearly written manuscript. The results of this work will be interesting not only for ribosomologists but to a broad “readership”.
Author Response
We are glad that both reviewers found our manuscript interesting and worthy publishing in IJMS. We have fixed all the typos found by the reviewers. Following their advice, we also added new Figure 5 that summarizes our findings.
We hope that our manuscript is now ready for publication.