Anti-Skin Inflammatory and Anti-Oxidative Effects of the Neoflavonoid Latifolin Isolated from Dalbergia odorifera in HaCaT and BJ-5ta Cells
Abstract
:1. Introduction
2. Results
2.1. Effects of Latifolin on Secretion of IL-6, IL-8, MDC, and RANTES in TNF-α/IFN-γ-Treated HaCaT Cells
2.2. Effects of Latifolin on TNF-α/IFN-γ-Treated ICAM-1 Expression
2.3. Effects of Latifolin on JAK2/STAT1(3) Pathway in HaCaT Cells
2.4. Effects of Latifolin on NF-κB Signaling Pathways in HaCaT Cells
2.5. Effects of Latifolin on Cell Viability in t-BHP-Induced BJ-5ta Cells
2.6. Effects of Latifolin on ROS Production Induced by t-BHP in BJ-5ta Cells
2.7. Effects of Latifolin on Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways in BJ-5ta Cells
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Isolation of Latifolin
4.2. The Structure Identification of Latifolin
4.3. Cell Culture and Reagents
4.4. MTT Assay
4.5. IL-6, IL-8, MDC, and RANTES Detection in Cell Supernatant
4.6. Extraction of Total, Nuclear, and Cytosolic Protein
4.7. Western Blot Analysis
4.8. Immunofluorescence Analysis
4.9. ROS Staining
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Dong, L.; Lee, H.; Liu, Z.; Lee, D.-S. Anti-Skin Inflammatory and Anti-Oxidative Effects of the Neoflavonoid Latifolin Isolated from Dalbergia odorifera in HaCaT and BJ-5ta Cells. Int. J. Mol. Sci. 2023, 24, 7371. https://doi.org/10.3390/ijms24087371
Dong L, Lee H, Liu Z, Lee D-S. Anti-Skin Inflammatory and Anti-Oxidative Effects of the Neoflavonoid Latifolin Isolated from Dalbergia odorifera in HaCaT and BJ-5ta Cells. International Journal of Molecular Sciences. 2023; 24(8):7371. https://doi.org/10.3390/ijms24087371
Chicago/Turabian StyleDong, Linsha, Hwan Lee, Zhiming Liu, and Dong-Sung Lee. 2023. "Anti-Skin Inflammatory and Anti-Oxidative Effects of the Neoflavonoid Latifolin Isolated from Dalbergia odorifera in HaCaT and BJ-5ta Cells" International Journal of Molecular Sciences 24, no. 8: 7371. https://doi.org/10.3390/ijms24087371