Linderone Isolated from Lindera erythrocarpa Exerts Antioxidant and Anti-Neuroinflammatory Effects via NF-κB and Nrf2 Pathways in BV2 and HT22 Cells
Abstract
:1. Introduction
2. Results
2.1. Effects of Linderone on BV2 and HT22 Cell Viability
2.2. Effects of Linderone on Inflammatory Factors in BV2 Cells
2.3. Effects of Linderone on Regulation of NF-κB (p65) Pathway in BV2 Cells
2.4. Effects of Linderone on Glutamate-Induced Oxidative Stress in HT22 Cells
2.5. Effects of Linderone on Nrf2/HO-1 Pathway in HT22 and BV2 Cells
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture and Cell Viability Assays
4.3. Measurement of NO Generation
4.4. PGE2 Assay
4.5. Assays for IL-6 and TNF-α
4.6. Western Blotting Analysis
4.7. p65 Localization
4.8. Determination of ROS Levels in HT22 Cells
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, Z.; Yoon, C.-S.; Lee, H.; Lee, H.-K.; Lee, D.-S. Linderone Isolated from Lindera erythrocarpa Exerts Antioxidant and Anti-Neuroinflammatory Effects via NF-κB and Nrf2 Pathways in BV2 and HT22 Cells. Int. J. Mol. Sci. 2023, 24, 7569. https://doi.org/10.3390/ijms24087569
Liu Z, Yoon C-S, Lee H, Lee H-K, Lee D-S. Linderone Isolated from Lindera erythrocarpa Exerts Antioxidant and Anti-Neuroinflammatory Effects via NF-κB and Nrf2 Pathways in BV2 and HT22 Cells. International Journal of Molecular Sciences. 2023; 24(8):7569. https://doi.org/10.3390/ijms24087569
Chicago/Turabian StyleLiu, Zhiming, Chi-Su Yoon, Hwan Lee, Hyeong-Kyu Lee, and Dong-Sung Lee. 2023. "Linderone Isolated from Lindera erythrocarpa Exerts Antioxidant and Anti-Neuroinflammatory Effects via NF-κB and Nrf2 Pathways in BV2 and HT22 Cells" International Journal of Molecular Sciences 24, no. 8: 7569. https://doi.org/10.3390/ijms24087569