Deregulation of the miR-19b/PPP2R5E Signaling Axis Shows High Functional Impact in Colorectal Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. MiR-19b Plays a Relevant Role in Regulating the Cell Viability of CRC Cells
2.2. Significant Relevance of miR-19b-Mediated PP2A Activity Inhibition through the Direct Negative Regulation of PPP2R5E
2.3. MiR-19b Promotes Cell Proliferation in a PPP2R5E-Dependent Manner
2.4. MiR-19b Regulates Cell Migration and Induces Colonosphere Formation in CRC Cells
2.5. MiR-19b/PPP2R5E Axis Regulates 5-FU Resistance in CRC Cells
3. Discussion
4. Materials and Methods
4.1. Cell Cultures and Transfection
4.2. Protein Extraction
4.3. Western Blot Analysis
4.4. Luciferase Assay
4.5. PP2A Phosphatase Activity Assay
4.6. Cell Viability Assay
4.7. Transwell Migration Assay
4.8. Colonosphere Formation
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Santos, A.; Cristóbal, I.; Caramés, C.; Luque, M.; Sanz-Álvarez, M.; Madoz-Gúrpide, J.; Rojo, F.; García-Foncillas, J. Deregulation of the miR-19b/PPP2R5E Signaling Axis Shows High Functional Impact in Colorectal Cancer Cells. Int. J. Mol. Sci. 2023, 24, 7779. https://doi.org/10.3390/ijms24097779
Santos A, Cristóbal I, Caramés C, Luque M, Sanz-Álvarez M, Madoz-Gúrpide J, Rojo F, García-Foncillas J. Deregulation of the miR-19b/PPP2R5E Signaling Axis Shows High Functional Impact in Colorectal Cancer Cells. International Journal of Molecular Sciences. 2023; 24(9):7779. https://doi.org/10.3390/ijms24097779
Chicago/Turabian StyleSantos, Andrea, Ion Cristóbal, Cristina Caramés, Melani Luque, Marta Sanz-Álvarez, Juan Madoz-Gúrpide, Federico Rojo, and Jesús García-Foncillas. 2023. "Deregulation of the miR-19b/PPP2R5E Signaling Axis Shows High Functional Impact in Colorectal Cancer Cells" International Journal of Molecular Sciences 24, no. 9: 7779. https://doi.org/10.3390/ijms24097779