SerpinA3K Deficiency Reduces Oxidative Stress in Acute Kidney Injury
Abstract
:1. Introduction
2. Results
2.1. Characterization of SerpinA3K Deficiency in Mice
2.2. Impact of SerpinA3K Deficiency during AKI
2.3. Histopathological Evaluation
2.4. Renal Location of SerpinA3K and Renal Damage Evaluation of Acute Kidney Injury
2.5. Inflammatory Response during AKI in SerpinA3K Deficiency
2.6. Response to Oxidative Stress in SerpinA3K Deficient Mice
3. Discussion
4. Material and Methods
4.1. Mouse Kidney Bilateral Renal Ischemia Model
4.2. Glomerular Filtration Rate Measurement
4.3. Histological Assessment of Tubular Injury
4.4. SerpinA3K Immunofluorescence
4.5. Urinary Hydrogen Peroxide Excretion
4.6. Serum and Urine Creatinine
4.7. Serum BUN, Sodium, and Potassium
4.8. Western Blot
4.9. mRNA Levels Assessed by Semiquantitative RT-PCR
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Target Genes | Probe gen Catalog Number |
---|---|
Hif1a | Mm00468869_m1 |
Gpx1 | Mm00656767_g1 |
Sod2 | Mm01313000_m1 |
Tnfa | Mm00443258_m1 |
Il6 | Mm00446190_m1 |
Tfgb1 | Mm01178820_m1 |
Il10 | Mm01288386_m1 |
Ccl2 | Rn00580555_m1 |
Nfe2l2 | Rn00582415_m1 |
Vegfa | Rn01511602_m1 |
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González-Soria, I.; Soto-Valadez, A.D.; Martínez-Rojas, M.A.; Ortega-Trejo, J.A.; Pérez-Villalva, R.; Gamba, G.; Sánchez-Navarro, A.; Bobadilla, N.A. SerpinA3K Deficiency Reduces Oxidative Stress in Acute Kidney Injury. Int. J. Mol. Sci. 2023, 24, 7815. https://doi.org/10.3390/ijms24097815
González-Soria I, Soto-Valadez AD, Martínez-Rojas MA, Ortega-Trejo JA, Pérez-Villalva R, Gamba G, Sánchez-Navarro A, Bobadilla NA. SerpinA3K Deficiency Reduces Oxidative Stress in Acute Kidney Injury. International Journal of Molecular Sciences. 2023; 24(9):7815. https://doi.org/10.3390/ijms24097815
Chicago/Turabian StyleGonzález-Soria, Isaac, Axel D. Soto-Valadez, Miguel Angel Martínez-Rojas, Juan Antonio Ortega-Trejo, Rosalba Pérez-Villalva, Gerardo Gamba, Andrea Sánchez-Navarro, and Norma A. Bobadilla. 2023. "SerpinA3K Deficiency Reduces Oxidative Stress in Acute Kidney Injury" International Journal of Molecular Sciences 24, no. 9: 7815. https://doi.org/10.3390/ijms24097815