Honey’s Yeast—New Source of Valuable Species for Industrial Applications
Abstract
:1. Introduction
2. Results and Discussion
2.1. Yeast Isolation and Identification
2.2. Growth of Yeasts Isolated from Honey on Different Carbon Sources
2.3. Value-Added Chemical Synthesis Using Yeast Isolated from Honey in Shake-Flask Screening
2.4. Bioreactor Studies
3. Materials and Methods
3.1. Honey Sample
3.2. Microorganism Isolation
3.3. Yeast Identification
3.4. API®/ID32
3.5. MALDI TOF/MS
3.6. rDNA Sequence-Based Identification
3.7. Microcultures
3.8. Media Compositions for Shake-Flask and Bioreactor Experiments
3.9. Culture Conditions for Inoculation, Shake-Flask and Bioreactor Experiments
3.10. Analytical Methods
3.11. Fermentation Parameter Calculations
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Strain No. | Color | Phenotype |
---|---|---|
1 | light beige | wrinkled |
2 | light beige | wrinkled |
3 | light beige | wrinkled |
4 | light beige | wrinkled |
5 | light beige | wrinkled |
6 | light beige | wrinkled |
7 | light beige | wrinkled |
8 | light beige | smooth |
9 | light beige | wrinkled |
10 | light beige | smooth |
11 | light beige | wrinkled |
12 | light beige | wrinkled |
13 | light beige | wrinkled |
14 | light beige | wrinkled |
15 | light beige | smooth |
Strain No. | Identification Profile of the Microorganism | ||||
---|---|---|---|---|---|
API 32C Test | MALDI TOF/MS Identification | rDNA Sequence-Based Identification | |||
Species | The Degree of Identification, % | Species | Identification Index Value a, % | Species | |
1 | Candida lipolytica | 99.9 | Yarrowia lipolytica | 1.90 | Yarrowia lipolytica |
2 | Candida lipolytica | 99.9 | Yarrowia lipolytica | 2.12 | Yarrowia lipolytica |
3 | Candida lipolytica | 99.9 | Yarrowia lipolytica | 2.00 | Yarrowia lipolytica |
4 | Candida lipolytica | 99.9 | Yarrowia lipolytica | 2.14 | Yarrowia lipolytica |
5 | Candida lipolytica | 99.9 | Yarrowia lipolytica | 1.99 | Yarrowia lipolytica |
6 | Candida lipolytica | 99.9 | Yarrowia lipolytica | 2.09 | Yarrowia lipolytica |
7 | Candida lipolytica | 99.9 | Yarrowia lipolytica | 2.18 | Yarrowia lipolytica |
8 | Candida magnoliae | 86.4 | Candida magnoliae | 2.13 | Candida magnoliae |
Candida globosa | 13.4 | ||||
9 | Candida lipolytica | 99.9 | Yarrowia lipolytica | 2.19 | Yarrowia lipolytica |
10 | Candida magnoliae | 98.7 | Candida magnoliae | 2.14 | Candida magnoliae |
11 | Candida lipolytica | 98.8 | Yarrowia lipolytica | 2.13 | Yarrowia lipolytica |
12 | Candida lipolytica | 99.9 | Yarrowia lipolytica | 1.98 | Yarrowia lipolytica |
13 | Candida lipolytica | 99.9 | Yarrowia lipolytica | 1.91 | Yarrowia lipolytica |
14 | Candida lipolytica | 99.9 | Yarrowia lipolytica | 1.97 | Yarrowia lipolytica |
15 | Candida magnoliae | 99.3 | Candida magnoliae | 1.86 | Starmerella magnoliae |
Metabolites | Strain No. | QX | Max. Concentration of Metabolite | QMET | YMET |
---|---|---|---|---|---|
g/L·h | g/L | mg/L·h a g/L h | mg/g b g/g | ||
Kynurenic Acid Production in Bioreactor | |||||
Kynurenic acid | 12 | 0.10 | 3.9 | 0.02 a | 0.1 |
Erythritol production in bioreactor | |||||
Erythritol | 9 | 0.22 | 32.6 | 0.34 | 0.33 |
Arabitol | 1.3 | 0.01 | 0.01 | ||
Mannitol | 4.8 | 0.05 | 0.05 | ||
Citric acid | 1.1 | 0.01 | 0.01 | ||
Mannitol production in bioreactor | |||||
Mannitol | 5 | 0.21 | 15.1 | 0.16 | 0.15 |
Arabitol | 1.6 | 0.01 | 0.02 | ||
Citric acid | 30.2 | 0.25 | 0.30 | ||
Citric acid production in bioreactor | |||||
Citric acid | 3 | 0.10 | 75.7 | 0.63 | 0.76 |
Arabitol | 2.05 | 0.17 | 0.02 | ||
Erythritol | 4.05 | 0.03 | 0.04 | ||
Mannitol | 7.15 | 0.06 | 0.07 |
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Ziuzia, P.; Janiec, Z.; Wróbel-Kwiatkowska, M.; Lazar, Z.; Rakicka-Pustułka, M. Honey’s Yeast—New Source of Valuable Species for Industrial Applications. Int. J. Mol. Sci. 2023, 24, 7889. https://doi.org/10.3390/ijms24097889
Ziuzia P, Janiec Z, Wróbel-Kwiatkowska M, Lazar Z, Rakicka-Pustułka M. Honey’s Yeast—New Source of Valuable Species for Industrial Applications. International Journal of Molecular Sciences. 2023; 24(9):7889. https://doi.org/10.3390/ijms24097889
Chicago/Turabian StyleZiuzia, Patrycja, Zuzanna Janiec, Magdalena Wróbel-Kwiatkowska, Zbigniew Lazar, and Magdalena Rakicka-Pustułka. 2023. "Honey’s Yeast—New Source of Valuable Species for Industrial Applications" International Journal of Molecular Sciences 24, no. 9: 7889. https://doi.org/10.3390/ijms24097889