Soybean (Glycine max L.) Lipoxygenase 1 (LOX 1) Is Modulated by Nitric Oxide and Hydrogen Sulfide: An In Vitro Approach
Abstract
:1. Introduction
2. Results and Discussion
2.1. Soybean LOX 1 Activity Is Downregulated by H2S and NO Donors
2.2. Soybean LOX 1 Activity Is Also Negatively Modulated by Tyrosine Nitration
2.3. Analysis of the Structure of Soybean LOX 1
3. Materials and Methods
3.1. Plant Material
3.2. Enzymatic LOX Activity and Protein Assay
3.3. In Vitro Treatment with Hydrogen Sulfide (H2S) and Nitric Oxide (NO) Donors, and Peroxynitrite (ONOO−)
3.4. SDS-PAGE and Immunoblot Analyses
3.5. Identification of Nitrated Tyrosine in Soybean LOX 1 Using Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
3.6. In Silico Analyses
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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# | Throughput | Length (Å) | Curvature 1 | Bottleneck (Å) 2 | Comments |
---|---|---|---|---|---|
#1 | 0.39 | 25.8 | 1.5 | 1.0 | |
#2 | 0.33 | 25.9 | 1.3 | 1.1 | |
#3 | 0.33 | 21.6 | 1.3 | 0.9 | |
#4 | 0.27 | 30.2 | 1.5 | 1.0 | C492 (at bottleneck) |
#5 | 0.26 | 41.3 | 2.0 | 1.0 | C127 |
#6 | 0.22 | 46.3 | 2.1 | 1.0 | C127 |
#7 | 0.16 | 41.2 | 1.5 | 1.0 | C492 |
#8 | 0.17 | 60.6 | 1.9 | 1.0 | C492 |
#9 | 0.16 | 42.3 | 1.7 | 1.0 | C127 |
#10 | 0.13 | 60.6 | 1.7 | 0.9 | C127 |
#11 | 0.12 | 41.5 | 1.8 | 0.9 | |
#12 | 0.09 | 47.8 | 1.5 | 1.0 | C492 (at bottleneck) |
#13 | 0.09 | 49.4 | 1.5 | 1.0 | C492 |
#14 | 0.07 | 75.0 | 2.6 | 0.9 | C127 |
#15 | 0.05 | 68.8 | 3.3 | 0.9 | C127 |
#16 | 0.01 | 88.3 | 4.8 | 0.9 | C492 |
#17 | 0.00 | 97.8 | 3.4 | 0.9 | C357C492 |
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González-Gordo, S.; López-Jaramillo, J.; Palma, J.M.; Corpas, F.J. Soybean (Glycine max L.) Lipoxygenase 1 (LOX 1) Is Modulated by Nitric Oxide and Hydrogen Sulfide: An In Vitro Approach. Int. J. Mol. Sci. 2023, 24, 8001. https://doi.org/10.3390/ijms24098001
González-Gordo S, López-Jaramillo J, Palma JM, Corpas FJ. Soybean (Glycine max L.) Lipoxygenase 1 (LOX 1) Is Modulated by Nitric Oxide and Hydrogen Sulfide: An In Vitro Approach. International Journal of Molecular Sciences. 2023; 24(9):8001. https://doi.org/10.3390/ijms24098001
Chicago/Turabian StyleGonzález-Gordo, Salvador, Javier López-Jaramillo, José M. Palma, and Francisco J. Corpas. 2023. "Soybean (Glycine max L.) Lipoxygenase 1 (LOX 1) Is Modulated by Nitric Oxide and Hydrogen Sulfide: An In Vitro Approach" International Journal of Molecular Sciences 24, no. 9: 8001. https://doi.org/10.3390/ijms24098001