Diversity of Cationic Antimicrobial Peptides in Black Cumin (Nigella sativa L.) Seeds
Abstract
:1. Introduction
2. Results
2.1. Proteomic Analysis
2.2. Peptide Isolation and Activity
2.2.1. Novel Defensin NsD4: Isolation and Structure Identification
2.2.2. Isolation and Identification of Novel Lipid-Transfer Proteins NsLTP2 and NsLTP3
2.3. NsLTP3 Antimicrobial Activity
2.3.1. Antifungal Activity
2.3.2. Antioomycetal Activity
2.3.3. Antibacterial Activity
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Peptide Extraction
4.3. Affinity Chromatography
4.4. Cation Exchange Chromatography
4.5. Reversed-Phase HPLC
4.6. MALDI-TOF/TOF MS
4.7. 3D Structure Modeling
4.8. Edman Sequencing
4.9. Reduction and Alkylation
4.10. Chemicals
4.11. Proteome Analysis
4.12. Antimicrobial Assay
4.12.1. Microorganisms
4.12.2. Antibacterial Assay
4.12.3. Antifungal Activity In Vitro
4.12.4. Antioomycetal Activity Ex Vivo
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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NsLTP3 Concentration, µM | Time of Incubation, h | ||
---|---|---|---|
96 | 120 | 144 | |
4.2 | +++/+++ | ++/++ | +/++ |
2.1 | ++/+++ | ++/++ | +/+ |
1.05 | ++/+++ | ++/++ | −/+ |
0.525 | ++/++ | +/+ | −/+ |
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Barashkova, A.S.; Smirnov, A.N.; Zorina, E.S.; Rogozhin, E.A. Diversity of Cationic Antimicrobial Peptides in Black Cumin (Nigella sativa L.) Seeds. Int. J. Mol. Sci. 2023, 24, 8066. https://doi.org/10.3390/ijms24098066
Barashkova AS, Smirnov AN, Zorina ES, Rogozhin EA. Diversity of Cationic Antimicrobial Peptides in Black Cumin (Nigella sativa L.) Seeds. International Journal of Molecular Sciences. 2023; 24(9):8066. https://doi.org/10.3390/ijms24098066
Chicago/Turabian StyleBarashkova, Anna S., Alexey N. Smirnov, Elena S. Zorina, and Eugene A. Rogozhin. 2023. "Diversity of Cationic Antimicrobial Peptides in Black Cumin (Nigella sativa L.) Seeds" International Journal of Molecular Sciences 24, no. 9: 8066. https://doi.org/10.3390/ijms24098066