Promotion of Colitis in B Cell-Deficient C57BL/6 Mice Infected with Enterotoxigenic Bacteroides fragilis
Abstract
:1. Introduction
2. Results
2.1. ETBF Infection Increases Indirect Parameters of Colon Inflammation in B-Cell-Deficient Mice
2.2. Persistent Inflammation in Large Intestines of ETBF-Infected B-Cell-Deficient Mice
2.3. Elevated Inflammatory Mediators in Cecum of B-Cell-Deficient Mice
2.4. ETBF Does Not Induce Inflammation in the Small Intestine
2.5. Th17/γδ T and Treg Response in ETBF-Infected B-Cell-Deficient Mice
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains
4.2. Mouse Experiments
4.3. Hematoxylin and Eosin (H&E) Staining
4.4. Histological Evaluation of Inflammation in the Colon and Ileum
4.5. Bacterial Colonization
4.6. RNA Extraction
4.7. Quantitative Real-Time PCR (qRT-PCR)
4.8. Flow Cytometry
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jo, M.; Hwang, S.; Lee, C.-G.; Hong, J.-E.; Kang, D.-H.; Yoo, S.-H.; Kim, W.-S.; Yoo, J.-Y.; Rhee, K.-J. Promotion of Colitis in B Cell-Deficient C57BL/6 Mice Infected with Enterotoxigenic Bacteroides fragilis. Int. J. Mol. Sci. 2024, 25, 364. https://doi.org/10.3390/ijms25010364
Jo M, Hwang S, Lee C-G, Hong J-E, Kang D-H, Yoo S-H, Kim W-S, Yoo J-Y, Rhee K-J. Promotion of Colitis in B Cell-Deficient C57BL/6 Mice Infected with Enterotoxigenic Bacteroides fragilis. International Journal of Molecular Sciences. 2024; 25(1):364. https://doi.org/10.3390/ijms25010364
Chicago/Turabian StyleJo, Minjeong, Soonjae Hwang, Chang-Gun Lee, Ju-Eun Hong, Da-Hye Kang, Sang-Hyeon Yoo, Woo-Seung Kim, Jung-Yoon Yoo, and Ki-Jong Rhee. 2024. "Promotion of Colitis in B Cell-Deficient C57BL/6 Mice Infected with Enterotoxigenic Bacteroides fragilis" International Journal of Molecular Sciences 25, no. 1: 364. https://doi.org/10.3390/ijms25010364