Over-Production of the Human SLC7A10 in E. coli and Functional Assay in Proteoliposomes
Abstract
:1. Introduction
2. Results
2.1. Expression in E. coli and Purification of hASC-1 Transporter
2.2. Reconstitution in Proteoliposomes of hASC-1
3. Discussion
3.1. Expression of ASC-1
3.2. Reconstitution of ASC-1
4. Materials and Methods
4.1. Cloning of Human hASC-1
4.2. Expression of Human ASC-1 Transporter
Effect of O2 on Protein Expression
4.3. Protein Quantification and Analysis
4.4. Purification of the hASC-1 Protein
4.5. Reconstitution of hASC-1 in Proteoliposomes
4.6. Transport Measurements in Proteoliposomes Reconstituted with hASC-1
4.7. Western Blotting
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Galluccio, M.; Mazza, T.; Scalise, M.; Tripicchio, M.; Scarpelli, M.; Tolomeo, M.; Pochini, L.; Indiveri, C. Over-Production of the Human SLC7A10 in E. coli and Functional Assay in Proteoliposomes. Int. J. Mol. Sci. 2024, 25, 536. https://doi.org/10.3390/ijms25010536
Galluccio M, Mazza T, Scalise M, Tripicchio M, Scarpelli M, Tolomeo M, Pochini L, Indiveri C. Over-Production of the Human SLC7A10 in E. coli and Functional Assay in Proteoliposomes. International Journal of Molecular Sciences. 2024; 25(1):536. https://doi.org/10.3390/ijms25010536
Chicago/Turabian StyleGalluccio, Michele, Tiziano Mazza, Mariafrancesca Scalise, Martina Tripicchio, Martina Scarpelli, Maria Tolomeo, Lorena Pochini, and Cesare Indiveri. 2024. "Over-Production of the Human SLC7A10 in E. coli and Functional Assay in Proteoliposomes" International Journal of Molecular Sciences 25, no. 1: 536. https://doi.org/10.3390/ijms25010536