Exploring the Multiple Roles of Notch1 in Biological Development: An Analysis and Study Based on Phylogenetics and Transcriptomics
Abstract
:1. Introduction
2. Results
2.1. Phylogenetic Analysis of Notch1
2.1.1. Identification and Evolutionary Analysis of the Notch Members in the Genome Database of the Notch Gene Family
2.1.2. Analysis of Collinearity and Gene Structure of the Notch1 Gene in the Lamprey
2.2. Tissue Distribution, Immune Response, and siRNA-Mediated Specific Gene Silencing
2.2.1. Distribution of Lr. Notch1 in Various Tissues of L. reissneri and siRNA-Mediated Specific Gene Silencing
2.2.2. The Immune Response to Various Stimuli Mediated by Lr. Notch1
2.3. Analysis of Transcriptional Data from Lr. Notch1 Gene Silencing
2.3.1. Enrichment Analysis of Differentially Expressed Genes
2.3.2. Validation of RNA-Seq Results by qPCR
3. Discussion
4. Materials and Methods
4.1. Experimental Animals
4.2. Multiple Sequence Alignment and Phylogenetic Analysis
4.3. Conservation Domain Prediction, Tertiary Structure Prediction
4.4. Gene Structure, Collinearity, and Motif Analysis
4.5. Real-Time Quantitative Polymerase Chain Reaction (qPCR) and Tissue Distribution Analysis, as Well as Determination of Immune Response to Different Stimuli
4.6. siRNA Target Design, Injection, and Silencing Validation
4.7. Transcriptome Sequencing and Data Analysis
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhou, Y.; Yan, Z.; Pang, Y.; Jiang, Y.; Zhuang, R.; Zhang, S.; Nurmamat, A.; Xiu, M.; Li, D.; Zhao, L.; et al. Exploring the Multiple Roles of Notch1 in Biological Development: An Analysis and Study Based on Phylogenetics and Transcriptomics. Int. J. Mol. Sci. 2024, 25, 611. https://doi.org/10.3390/ijms25010611
Zhou Y, Yan Z, Pang Y, Jiang Y, Zhuang R, Zhang S, Nurmamat A, Xiu M, Li D, Zhao L, et al. Exploring the Multiple Roles of Notch1 in Biological Development: An Analysis and Study Based on Phylogenetics and Transcriptomics. International Journal of Molecular Sciences. 2024; 25(1):611. https://doi.org/10.3390/ijms25010611
Chicago/Turabian StyleZhou, Yuesi, Zihao Yan, Ya Pang, Yao Jiang, Ruyu Zhuang, Shuyuan Zhang, Ayqeqan Nurmamat, Min Xiu, Ding Li, Liang Zhao, and et al. 2024. "Exploring the Multiple Roles of Notch1 in Biological Development: An Analysis and Study Based on Phylogenetics and Transcriptomics" International Journal of Molecular Sciences 25, no. 1: 611. https://doi.org/10.3390/ijms25010611